Mouse DMPO Monoclonal Antibody

DMPO (5,5-dimethyl-2-(8-octanoic acid)-1-pyrroline-N-oxide)

Cat. Num. AAA6011180

• Applications: ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: DMPO; Monoclonal Antibody; DMPO (5; 5-dimethyl-2-(8-octanoic acid)-1-pyrroline-N-oxide); Anti -DMPO (5; anti-DMPO antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: N1664A
• Specificity: Recognzies chicken DMPO at ~90kD. Reacts with DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in TBS, pH 7.4, 50% glycerol.

• Applicable Applications for anti-DMPO antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry.; Dilution: ELISA: 1-10ug/ml; Immunocytochemistry: 10ug/ml; Immunoprecipitation: 25ug
• Immunogen: 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide, conjugated to Ovalbumin.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunofluorescence (IF)

(IF analysis of DMPO in mouse macrophage cell lines)

Western Blot (WB)

(Western blot analysis of DMPO)

Related Product Information for anti-DMPO antibody

The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states (1). The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems (2-6). Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals (1). DMPO adducts can be generated with protein and DNA radicals (7).

Product Categories/Family for anti-DMPO antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 51242284
• NCBI Official Full Name: DmpO
• Protein Family: Phenol hydroxylase P4 protein