Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Dihydrolipoyl Transacetylase (DLAT) Standard Curve.)
Rat Dihydrolipoyl Transacetylase (DLAT) ELISA Kit | DLAT elisa kit
Rat Dihydrolipoyl Transacetylase (DLAT) ELISA Kit
Reactivity
Rat
Synonyms
Dihydrolipoyl Transacetylase (DLAT); Rat Dihydrolipoyl Transacetylase (DLAT) ELISA Kit; DLAT; DLTA; PDC-E2; PDCE2; 70 kDa mitochondrial autoantigen of primary biliary cirrhosis; E2 component of pyruvate dehydrogenase complex; M2 antigen complex 70 kDa subunit; DLAT elisa kit
Reactivity
Rat
Specificity
Rat Dihydrolipoyl Transacetylase (DLAT) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DLAT. No significant cross-reactivity or interference between Rat DLAT and analogues was observed.
Sequence Length
632
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Dihydrolipoyl Transacetylase (DLAT) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Dihydrolipoyl Transacetylase (DLAT) Standard Curve.)
Related Product Information for DLAT elisa kit
Description: This Rat Dihydrolipoyl Transacetylase (DLAT) ELISA Kit employs a two-site sandwich ELISA to quantitate DLAT in samples. An antibody specific for DLAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DLAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLAT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLAT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Dihydrolipoyl transacetylase is the component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure.
Background: Dihydrolipoyl transacetylase is the component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
NCBI Official Synonym Full Names
dihydrolipoamide S-acetyltransferase
NCBI Official Symbol
Dlat
NCBI Protein Information
dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
UniProt Protein Name
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
Protein Family
UniProt Gene Name
Dlat
UniProt Synonym Gene Names
PBC; PDC-E2; PDCE2
UniProt Entry Name
ODP2_RAT
NCBI Description
putative dihydrolipoamide acetyltransferase [RGD, Feb 2006]
Similar Products
Product Notes
The Rat DLAT dlat (Catalog #AAA9712431) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712431 ELISA Kit recognizes Rat DLAT. It is sometimes possible for the material contained within the vial of "Dihydrolipoyl Transacetylase (DLAT), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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