Immunohistochemistry (IHC)
(MBS9602963 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human GRIK5 Polyclonal Antibody | anti-GRIK5 antibody
GRIK5 Antibody
Gene Names
GRIK5; KA2; EAA2; GRIK2; GluK5
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
GRIK5; Polyclonal Antibody; GRIK5 Antibody; EAA 2; EAA2; Excitatory amino acid receptor 2; GluK5; GluRgamma2; Glutamate receptor; Glutamate receptor KA 2; Glutamate receptor KA-2; Glutamate receptor KA2; ionotropic kainate 5 [Precursor]; ionotropic; kainate 5 (gamma 2); kainate 5; GRIK 2; GRIK 5; GRIK2; Grik5; GRIK5_HUMAN; iGlu5; ionotropic kainate 5; KA2; MGC118086; anti-GRIK5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
GRIK5 antibody detects endogenous levels of total GRIK5
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
981
Applicable Applications for anti-GRIK5 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
IHC: 1:200
Immunogen
A synthesized peptide derived from human GRIK5
Subcellular Location
Cell Membrane. Cell Junction > Synapse > Postsynaptic Cell Membrane.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Xenopus
Similarity
Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9602963 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9602963 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis GRIK5 using HepG2 whole cell lysates)
Western Blot (WB)
(Western blot analysis GRIK5 using HepG2 whole cell lysates)
Related Product Information for anti-GRIK5 antibody
Function: Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds kainate > quisqualate > domoate > L-glutamate > > AMPA > > NMDA = 1S, 3R-ACPD.
Subunit Structure: Tetramer of two or more different subunits. Associates with GRIK1 (both edited and unedited versions), GRIK2, or GRIK3 to form functional channels. Homomeric associations do not produce any channel activity (By similarity).
Similarity: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK5 subfamily.
Subunit Structure: Tetramer of two or more different subunits. Associates with GRIK1 (both edited and unedited versions), GRIK2, or GRIK3 to form functional channels. Homomeric associations do not produce any channel activity (By similarity).
Similarity: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK5 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 109 kDa
Predicted: 110 kDa
Predicted: 110 kDa
NCBI Official Full Name
glutamate receptor ionotropic, kainate 5 isoform 1
NCBI Official Synonym Full Names
glutamate ionotropic receptor kainate type subunit 5
NCBI Official Symbol
GRIK5
NCBI Official Synonym Symbols
KA2; EAA2; GRIK2; GluK5
NCBI Protein Information
glutamate receptor ionotropic, kainate 5
UniProt Protein Name
Glutamate receptor ionotropic, kainate 5
Protein Family
UniProt Gene Name
GRIK5
UniProt Synonym Gene Names
GRIK2; GluK5; EAA2; KA2
NCBI Description
This gene encodes a protein that belongs to the glutamate-gated ionic channel family. Glutamate functions as the major excitatory neurotransmitter in the central nervous system through activation of ligand-gated ion channels and G protein-coupled membrane receptors. The protein encoded by this gene forms functional heteromeric kainate-preferring ionic channels with the subunits encoded by related gene family members. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
Uniprot Description
Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds kainate > quisqualate > domoate > L-glutamate >> AMPA >> NMDA = 1S,3R-ACPD.
Research Articles on GRIK5
Similar Products
Product Notes
The GRIK5 grik5 (Catalog #AAA9602963) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GRIK5 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GRIK5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the GRIK5 grik5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GRIK5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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