Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using DIMT1 antibody.)
Rabbit DIMT1 Polyclonal Antibody | anti-DIMT1 antibody
DIMT1 Polyclonal Antibody
Gene Names
DIMT1; DIM1; DIMT1L; HUSSY5; HSA9761
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
DIMT1; Polyclonal Antibody; DIMT1 Polyclonal Antibody; Probable dimethyladenosine transferase; DIM1 dimethyladenosine transferase 1 homolog; DIM1 dimethyladenosine transferase 1-like; Probable 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase; Probable 18S rRNA dimethylase; Probable S-adenosylmethionine-6-N'; N'-adenosyl(rRNA) dimethyltransferase; DIMT1L; HUSSY-05; anti-DIMT1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
DIMT1 polyclonal antibody detects endogenous levels of DIMT1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
313
Applicable Applications for anti-DIMT1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:100
IHC: 1:50-1:100
Immunogen
Recombinant full length Human DIMT1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using DIMT1 antibody.)
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using DIMT1 antibody.)
Related Product Information for anti-DIMT1 antibody
DIMT1 (DIM1 dimethyladenosine transferase 1), also known as DIMT1L or HUSSY-05, is a 313 amino acid protein that belongs to the rRNA adenine N(6)-methyltransferase family. Localized to the nucleolus, DIMT1 functions to dimethylate adjacent adenosines on the conserved hairpin loop of 18S rRNA in the 40S particle. The gene encoding DIMT1 maps to chromosome 5, which contains 181 million base pairs and comprises nearly 6% of the human genome. Chromosome 5 is associated with Cockayne syndrome through the ERCC8 gene and familial adenomatous polyposis through the adenomatous polyposis coli (APC) tumor suppressor gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
~ 35kDa
NCBI Official Full Name
Probable dimethyladenosine transferase
NCBI Official Synonym Full Names
DIMT1 rRNA methyltransferase and ribosome maturation factor
NCBI Official Symbol
DIMT1
NCBI Official Synonym Symbols
DIM1; DIMT1L; HUSSY5; HSA9761
NCBI Protein Information
probable dimethyladenosine transferase
UniProt Protein Name
Probable dimethyladenosine transferase
Protein Family
UniProt Gene Name
DIMT1
UniProt Synonym Gene Names
DIMT1L
UniProt Entry Name
DIM1_HUMAN
NCBI Description
The protein encoded by this gene is a methyltransferase that is responsible for dimethylation of adjacent adenosines near the 18S rRNA decoding site. The encoded protein is essential for ribosome biogenesis, although its catalytic activity is not involved in the process. The yeast ortholog of this protein functions in the cytoplasm while this protein functions in the nucleus. [provided by RefSeq, Jan 2017]
Uniprot Description
DIMT1: Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle. Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.
Protein type: EC 2.1.1.183; Nucleolus; RNA-binding; Methyltransferase
Chromosomal Location of Human Ortholog: 5q12.1
Cellular Component: cytoplasm; nucleolus; nucleus
Molecular Function: rRNA (adenine-N6,N6-)-dimethyltransferase activity
Biological Process: rRNA methylation
Research Articles on DIMT1
Similar Products
DIMT1 Antibody
Host: Rabbit
Reactivity: Human, Mouse
Apps: Western Blot, Immunohistochemistry, ELISA
DIMT1 Antibody
Host: Rabbit
Reactivity: Human, Mouse
Apps: Western Blot, Immunohistochemistry, ELISA
Product Notes
The DIMT1 dimt1 (Catalog #AAA3012167) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DIMT1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DIMT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:100. Researchers should empirically determine the suitability of the DIMT1 dimt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DIMT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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