Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SKIL antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.)
Rabbit SKIL Polyclonal Antibody | anti-SKIL antibody
SKIL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SKIL antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SKIL antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
SKIL: May have regulatory role in cell division or differentiation in response to extracellular signals. Interacts with SMAD2, SMAD3 and RNF111. Isoform 1 interacts with WWP1. Isoform SNON and isoform SNOA are widely expressed. Highest expression is found in skeletal muscle, followed by placenta and lung. Lowest expression in heart, brain and pancreas. Isoform SNOI expression is restricted to skeletal muscle. Belongs to the SKI family. 5 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 3q26
Cellular Component: nucleoplasm; PML body; protein complex; cytoplasm; acrosome; nucleus
Molecular Function: protein domain specific binding; protein binding; DNA binding; protein complex binding; chromatin binding; SMAD binding; transcription corepressor activity
Biological Process: transcription initiation from RNA polymerase II promoter; skeletal muscle development; transcription, DNA-dependent; blastocyst formation; negative regulation of transcription from RNA polymerase II promoter; response to antibiotic; regulation of apoptosis; negative regulation of cell differentiation; transforming growth factor beta receptor signaling pathway; response to cytokine stimulus; positive regulation of axonogenesis; spermatogenesis; gene expression; negative regulation of transforming growth factor beta receptor signaling pathway; cell cycle arrest; lymphocyte homeostasis
Research Articles on SKIL
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Product Notes
The SKIL skil (Catalog #AAA9127041) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SKIL reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SKIL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the SKIL skil for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SKIL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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