Mouse anti-Human, Rat DIMT1L Polyclonal Antibody | anti-DIMT1 antibody

DIMT1L (HSA9761, Probable Dimethyladenosine Transferase, DIMT1, HUSSY-05, S-adenosylmethionine-6-N',N'-adenosyl(rRNA) Dimethyltransferase, 18S rRNA Dimethylase, DIM1 Dimethyladenosine Transferase 1-like, 18S rRNA (Adenine(1779)-N(6)/Adenine(1780)-N(6))-di

Cat. Num. AAA645957

• Gene Names: DIMT1; DIM1; DIMT1L; HUSSY5; HSA9761
• Reactivity: Human, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: DIMT1L; Polyclonal Antibody; DIMT1L (HSA9761; Probable Dimethyladenosine Transferase; DIMT1; HUSSY-05; S-adenosylmethionine-6-N'; N'-adenosyl(rRNA) Dimethyltransferase; 18S rRNA Dimethylase; DIM1 Dimethyladenosine Transferase 1-like; 18S rRNA (Adenine(1779)-N(6)/Adenine(1780)-N(6))-di; Anti -DIMT1L (HSA9761; anti-DIMT1 antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HSA9761. Species Crossreactivity: rat.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MPKVKSGAIGRRRGRQEQRRELKSAGGLMFNTGIGQHILKNPLIINSIIDKAALRPTDVVLEVGPGTGNMTVKLLEKAKKVVACELDPRLVAELHKRVQGTPVASKLQVLVGDVLKTDLPFFDTCVANLPYQISSPFVFKLLLHRPFFRCAILMFQREFALRLVAKPGDKLYCRLSINTQLLARVDHLMKVGKNNFRPPPKVESSVVRIEPKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSSAVQQLLEKNYRIHCSVHNIIIPEDFSIADKIQQILTSTGFSDKRARSMDIDDFIRLLHGFNAEGIHFS

• Applicable Applications for anti-DIMT1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot and Immunofluorescence.; Dilution: Immunofluorescence: 10ug/ml
• Immunogen: Full length human HSA9761, aa1-313 (NP_055288).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(HSA9761 polyclonal antibody. Western Blot analysis of HSA9761 expression in rat brain.)

Western Blot (WB)

(Western Blot analysis of HSA9761 expression in transfected 293T cell line by HSA9761 polyclonal antibody. Lane 1: HSA9761 transfected lysate (34.43kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of purified antibody to DIMT1L on HeLa cell. [antibody concentration 10ug/ml])

Related Product Information for anti-DIMT1 antibody

DIMT1L specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle.

Product Categories/Family for anti-DIMT1 antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 12803983
• NCBI GeneID: 27292
• UniProt Accession #: Q9UNQ2; O76025; Q9BU77; Q9UES1
• Molecular Weight: 35,236 Da
• NCBI Official Full Name: DIMT1L protein
• NCBI Official Synonym Full Names: DIM1 dimethyladenosine transferase 1 homolog (S. cerevisiae)
• NCBI Official Symbol: DIMT1
• NCBI Official Synonym Symbols: DIM1; DIMT1L; HUSSY5; HSA9761
• NCBI Protein Information: probable dimethyladenosine transferase; 18S rRNA dimethylase; probable 18S rRNA dimethylase; S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase; 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase; probable S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase; probable 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase
• UniProt Protein Name: Probable dimethyladenosine transferase
• Protein Family: Probable dimethyladenosine transferase
• UniProt Gene Name: DIMT1
• UniProt Synonym Gene Names: DIMT1L
• UniProt Entry Name: DIM1_HUMAN

Uniprot Description

Function: Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle

By similarity.

Catalytic activity: 4 S-adenosyl-L-methionine + adenine(1779)/adenine(1780) in 18S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1779)/N(6)-dimethyladenine(1780) in 18S rRNA.

Subcellular location: Nucleus › nucleolus Ref.5.

Sequence similarities: Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.

Sequence caution: The sequence AAC72947.1 differs from that shown. Reason: Contaminating sequence. Sequence of unknown origin in the N-terminal part.