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Western Blot (WB) (HSA9761 polyclonal antibody. Western Blot analysis of HSA9761 expression in rat brain.)

Mouse anti-Human, Rat DIMT1L Polyclonal Antibody | anti-DIMT1 antibody

DIMT1L (HSA9761, Probable Dimethyladenosine Transferase, DIMT1, HUSSY-05, S-adenosylmethionine-6-N',N'-adenosyl(rRNA) Dimethyltransferase, 18S rRNA Dimethylase, DIM1 Dimethyladenosine Transferase 1-like, 18S rRNA (Adenine(1779)-N(6)/Adenine(1780)-N(6))-di

Gene Names
DIMT1; DIM1; DIMT1L; HUSSY5; HSA9761
Reactivity
Human, Rat
Applications
Western Blot, Immunofluorescence
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
DIMT1L; Polyclonal Antibody; DIMT1L (HSA9761; Probable Dimethyladenosine Transferase; DIMT1; HUSSY-05; S-adenosylmethionine-6-N'; N'-adenosyl(rRNA) Dimethyltransferase; 18S rRNA Dimethylase; DIM1 Dimethyladenosine Transferase 1-like; 18S rRNA (Adenine(1779)-N(6)/Adenine(1780)-N(6))-di; Anti -DIMT1L (HSA9761; anti-DIMT1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human HSA9761. Species Crossreactivity: rat.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MPKVKSGAIGRRRGRQEQRRELKSAGGLMFNTGIGQHILKNPLIINSIIDKAALRPTDVVLEVGPGTGNMTVKLLEKAKKVVACELDPRLVAELHKRVQGTPVASKLQVLVGDVLKTDLPFFDTCVANLPYQISSPFVFKLLLHRPFFRCAILMFQREFALRLVAKPGDKLYCRLSINTQLLARVDHLMKVGKNNFRPPPKVESSVVRIEPKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSSAVQQLLEKNYRIHCSVHNIIIPEDFSIADKIQQILTSTGFSDKRARSMDIDDFIRLLHGFNAEGIHFS
Applicable Applications for anti-DIMT1 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
Suitable for use in Western Blot and Immunofluorescence.
Dilution: Immunofluorescence: 10ug/ml
Immunogen
Full length human HSA9761, aa1-313 (NP_055288).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(HSA9761 polyclonal antibody. Western Blot analysis of HSA9761 expression in rat brain.)

Western Blot (WB) (HSA9761 polyclonal antibody. Western Blot analysis of HSA9761 expression in rat brain.)

Western Blot (WB)

(Western Blot analysis of HSA9761 expression in transfected 293T cell line by HSA9761 polyclonal antibody. Lane 1: HSA9761 transfected lysate (34.43kD). Lane 2: Non-transfected lysate.)

Western Blot (WB) (Western Blot analysis of HSA9761 expression in transfected 293T cell line by HSA9761 polyclonal antibody. Lane 1: HSA9761 transfected lysate (34.43kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of purified antibody to DIMT1L on HeLa cell. [antibody concentration 10ug/ml])

Immunofluorescence (IF) (Immunofluorescence of purified antibody to DIMT1L on HeLa cell. [antibody concentration 10ug/ml])
Related Product Information for anti-DIMT1 antibody
DIMT1L specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle.
Product Categories/Family for anti-DIMT1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
35,236 Da
NCBI Official Full Name
DIMT1L protein
NCBI Official Synonym Full Names
DIM1 dimethyladenosine transferase 1 homolog (S. cerevisiae)
NCBI Official Symbol
DIMT1
NCBI Official Synonym Symbols
DIM1; DIMT1L; HUSSY5; HSA9761
NCBI Protein Information
probable dimethyladenosine transferase; 18S rRNA dimethylase; probable 18S rRNA dimethylase; S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase; 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase; probable S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase; probable 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase
UniProt Protein Name
Probable dimethyladenosine transferase
UniProt Gene Name
DIMT1
UniProt Synonym Gene Names
DIMT1L
UniProt Entry Name
DIM1_HUMAN

Uniprot Description

Function: Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle

By similarity.

Catalytic activity: 4 S-adenosyl-L-methionine + adenine(1779)/adenine(1780) in 18S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1779)/N(6)-dimethyladenine(1780) in 18S rRNA.

Subcellular location: Nucleus › nucleolus Ref.5.

Sequence similarities: Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.

Sequence caution: The sequence AAC72947.1 differs from that shown. Reason: Contaminating sequence. Sequence of unknown origin in the N-terminal part.

Similar Products

Product Notes

The DIMT1 dimt1 (Catalog #AAA645957) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DIMT1L (HSA9761, Probable Dimethyladenosine Transferase, DIMT1, HUSSY-05, S-adenosylmethionine-6-N',N'-adenosyl(rRNA) Dimethyltransferase, 18S rRNA Dimethylase, DIM1 Dimethyladenosine Transferase 1-like, 18S rRNA (Adenine(1779)-N(6)/Adenine(1780)-N(6))-di reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DIMT1L can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Suitable for use in Western Blot and Immunofluorescence. Dilution: Immunofluorescence: 10ug/ml. Researchers should empirically determine the suitability of the DIMT1 dimt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MPKVKSGAIG RRRGRQEQRR ELKSAGGLMF NTGIGQHILK NPLIINSIID KAALRPTDVV LEVGPGTGNM TVKLLEKAKK VVACELDPRL VAELHKRVQG TPVASKLQVL VGDVLKTDLP FFDTCVANLP YQISSPFVFK LLLHRPFFRC AILMFQREFA LRLVAKPGDK LYCRLSINTQ LLARVDHLMK VGKNNFRPPP KVESSVVRIE PKNPPPPINF QEWDGLVRIT FVRKNKTLSA AFKSSAVQQL LEKNYRIHCS VHNIIIPEDF SIADKIQQIL TSTGFSDKRA RSMDIDDFIR LLHGFNAEGI HFS. It is sometimes possible for the material contained within the vial of "DIMT1L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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