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Western Blot (WB) (Western blot analysis of Dim2 expression in mouse liver (A) whole cell lysates.)

Rabbit Dim2 Polyclonal Antibody | anti-Dim2 antibody

Anti-Dim2 Antibody

Gene Names
TXNL4B; DLP; Dim2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Purified by Immunogen Affinity Chromatography
Synonyms
Dim2; Polyclonal Antibody; Anti-Dim2 Antibody; DIM2; DLP; Thioredoxin-like protein 4B; Dim1-like protein; anti-Dim2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of Dim2 protein.
Purity/Purification
Purified by Immunogen Affinity Chromatography
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Applicable Applications for anti-Dim2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB: 1:500-1:2000
IF/ICC: 1:50-1:200
Immunogen
Recombinant fusion protein of human Dim2. The exact sequence is proprietary.
Preparation and Storage
Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of Dim2 expression in mouse liver (A) whole cell lysates.)

Western Blot (WB) (Western blot analysis of Dim2 expression in mouse liver (A) whole cell lysates.)

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(Immunofluorescent analysis of Dim2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)

Immunofluorescence (IF)/Immunocytochemistry (ICC) (Immunofluorescent analysis of Dim2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
Related Product Information for anti-Dim2 antibody
Rabbit polyclonal antibody to Dim2

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17,015 Da
NCBI Official Full Name
thioredoxin-like protein 4B
NCBI Official Synonym Full Names
thioredoxin-like 4B
NCBI Official Symbol
TXNL4B
NCBI Official Synonym Symbols
DLP; Dim2
NCBI Protein Information
thioredoxin-like protein 4B; Dim1-like protein
UniProt Protein Name
Thioredoxin-like protein 4B
UniProt Gene Name
TXNL4B
UniProt Synonym Gene Names
DIM2; DLP
UniProt Entry Name
TXN4B_HUMAN

Uniprot Description

TXNL4B: Essential role in pre-mRNA splicing. Required in cell cycle progression for S/G(2) transition. Belongs to the DIM1 family.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 16q22.2

Cellular Component: spliceosome; U4/U6 x U5 tri-snRNP complex; snRNP U5

Biological Process: mitosis; spliceosome assembly

Research Articles on Dim2

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Product Notes

The Dim2 txnl4b (Catalog #AAA8308533) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Dim2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Dim2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1:500-1:2000 IF/ICC: 1:50-1:200. Researchers should empirically determine the suitability of the Dim2 txnl4b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dim2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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