Rabbit anti-Human DHX58 Polyclonal Antibody | anti-DHX58 antibody

DHX58, NT (DHX58, D11LGP2E, LGP2, Probable ATP-dependent RNA helicase DHX58, Probable ATP-dependent helicase LGP2, Protein D11Lgp2 homolog, RIG-I-like receptor 3, RIG-I-like receptor LGP2) (APC)

Cat. Num. AAA6292125

• Gene Names: DHX58; LGP2; RLR-3; D11LGP2; D11lgp2e
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DHX58; Polyclonal Antibody; NT (DHX58; D11LGP2E; LGP2; Probable ATP-dependent RNA helicase DHX58; Probable ATP-dependent helicase LGP2; Protein D11Lgp2 homolog; RIG-I-like receptor 3; RIG-I-like receptor LGP2) (APC); RIG-I-like receptor LGP2; anti-DHX58 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-DHX58 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DHX58 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-77 amino acids from the N-terminal region of human DHX58.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(DHX58 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the DHX58 antibody detected the DHX58 protein (arrow).)

Immunohistochemistry (IHC)

(DHX58 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DHX58 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-DHX58 antibody

Antibodies; DNA Replication; Repair

References

Mosbruger, T.L., et al. J. Infect. Dis. 201(9):1371-1380(2010). Parisien, J.P., et al. J. Virol. 83(14):7252-7260(2009). Li, X., et al. J. Biol. Chem. 284(20):13881-13891(2009). Murali, A., et al. J. Biol. Chem. 283(23):15825-15833(2008). Saito, T., et al. Proc. Natl. Acad. Sci. U.S.A. 104(2):582-587(2007). Komuro, A., et al. J. Virol. 80(24):12332-12342(2006). Yoneyama, M., et al. J. Immunol. 175(5):2851-2858(2005). Cui, Y., et al. Genomics 78(3):129-134(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 149408122
• NCBI GeneID: 79132
• NCBI Accession #: NP_077024.2
• NCBI GenBank Nucleotide #: NM_024119.2
• UniProt Accession #: Q96C10; Q9HAM6
• Molecular Weight: 76,613 Da
• NCBI Official Full Name: probable ATP-dependent RNA helicase DHX58
• NCBI Official Synonym Full Names: DEXH (Asp-Glu-X-His) box polypeptide 58
• NCBI Official Symbol: DHX58
• NCBI Official Synonym Symbols: LGP2; RLR-3; D11LGP2; D11lgp2e
• NCBI Protein Information: probable ATP-dependent RNA helicase DHX58; RLR; RNA helicase LGP2; RIG-I-like receptor 3; protein D11Lgp2 homolog; RIG-I-like receptor LGP2; ortholog of mouse D11lgp2; probable ATP-dependent helicase LGP2
• UniProt Protein Name: Probable ATP-dependent RNA helicase DHX58
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DHX58
• UniProt Synonym Gene Names: D11LGP2E; LGP2; RLR-3; RLR
• UniProt Entry Name: DHX58_HUMAN

Uniprot Description

DHX58: Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1- dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate. Belongs to the helicase family. RLR subfamily.

Protein type: Helicase; EC 3.6.4.13; Inhibitor

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoplasm

Molecular Function: protein binding; single-stranded RNA binding; DNA binding; zinc ion binding; double-stranded RNA binding; helicase activity; ATP binding

Biological Process: viral reproduction; negative regulation of innate immune response; metabolic process; response to virus; positive regulation of interferon type I production; innate immune response; defense response to virus; negative regulation of interferon type I production; regulation of innate immune response