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Western Blot (WB) (Western blot analysis of MYOZAP in rat kidney tissue lysate with MYOZAP antibody at (A) 1 and (B) 2 μg/mL.)

Rabbit MYOZAP Polyclonal Antibody | anti-GCOM1 antibody

MYOZAP Antibody

Gene Names
GCOM1; gcom; Gcom2; MYZAP; GRINL1A; MYZAP-POLR2M
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
MYOZAP Antibody is affinity chromatography purified via peptide column.
Synonyms
MYOZAP; Polyclonal Antibody; MYOZAP Antibody; gcom; Gcom2; MYZAP; GRINL1A; MYZAP-POLR2M; Myocardial zonula adherens protein; GRINL1A upstream protein; Gup; GRINL1A complex locus; anti-GCOM1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Multiple isoforms of MYOZAP are known to exist.
Purity/Purification
MYOZAP Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
550
Applicable Applications for anti-GCOM1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
MYOZAP antibody can be used for detection of MYOZAP by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
MYOZAP antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human MYOZAP.
Buffer
MYOZAP Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
MYOZAP antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of MYOZAP in rat kidney tissue lysate with MYOZAP antibody at (A) 1 and (B) 2 μg/mL.)

Western Blot (WB) (Western blot analysis of MYOZAP in rat kidney tissue lysate with MYOZAP antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of MYOZAP in human lung tissue with MYOZAP antibody at 5 μg/mL.)

Immunohistochemistry (IHC) (Immunohistochemistry of MYOZAP in human lung tissue with MYOZAP antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of MYOZAP in human lung tissue with MYOZAP antibody at 20 μg/mL.)

Immunofluorescence (IF) (Immunofluorescence of MYOZAP in human lung tissue with MYOZAP antibody at 20 μg/mL.)
Related Product Information for anti-GCOM1 antibody
MYOZAP Antibody: MYOZAP, also known as GRINL1A, is a 54 kDa highly conserved cardiac protein. It is strongly expressed in the heart and lung and is a novel component of intercalated disc. MYOZAP interacts with myosin phosphatase-RhoA interacting protein (MRIP) and acts as an activator of Rho-dependent SRF signaling. Knockdown study in zebrafish results in cardiomyopathy with severe dysfunction. The MYOZAP gene is part of a complex transcript unit that includes the gene for glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (GRINL1A). Transcription of this gene occurs at an upstream promoter, with two different groups of alternatively spliced variants: Gup for GRINL1A upstream transcripts and Gcom for GRINL1A combined transcripts.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64,046 Da
NCBI Official Full Name
GRINL1A combined protein isoform 1
NCBI Official Synonym Full Names
GRINL1A complex locus 1
NCBI Official Symbol
GCOM1
NCBI Official Synonym Symbols
gcom; Gcom2; MYZAP; GRINL1A; MYZAP-POLR2M
NCBI Protein Information
GRINL1A combined protein; MYZAP-POLR2M protein; MYZAP-POLR2M readthrough; GRINL1A combined protein Gcom12; NMDAR1 subunit-interacting protein; myocardial zonula adherens protein; glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A combined protein
UniProt Protein Name
Myocardial zonula adherens protein
UniProt Gene Name
MYZAP
UniProt Synonym Gene Names
MYOZAP; Gup
UniProt Entry Name
MYZAP_HUMAN

NCBI Description

This locus represents naturally occurring readthrough transcription between the neighboring MYZAP (myocardial zonula adherens protein) and POLR2M (polymerase (RNA) II (DNA directed) polypeptide M) genes on chromosome 15. Alternative splicing results in multiple readthrough transcript variants. Readthrough variants may encode proteins that share sequence identity with the upstream gene product or with both the upstream and downstream gene products. Some readthrough transcript variants are also expected to be candidates for nonsense-mediated decay (NMD). [provided by RefSeq, Oct 2013]

Uniprot Description

GCOM1: Plays a role in cellular signaling via Rho-related GTP- binding proteins and subsequent activation of transcription factor SRF. Targets TJP1 to cell junctions. In cortical neurons, may play a role in glutaminergic signal transduction through interaction with the NMDA receptor subunit GRIN1. Belongs to the MYZAP family. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 15q21.3

Research Articles on GCOM1

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Product Notes

The GCOM1 myzap (Catalog #AAA151319) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MYOZAP Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MYOZAP can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). MYOZAP antibody can be used for detection of MYOZAP by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the GCOM1 myzap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYOZAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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