Human E3 ubiquitin-protein ligase AMFR ELISA Kit | AMFR elisa kit

Human E3 ubiquitin-protein ligase AMFR ELISA Kit

Cat. Num. AAA762273

• Gene Names: AMFR; GP78; RNF45
• Reactivity: Human
• Synonyms: E3 ubiquitin-protein ligase AMFR; Human E3 ubiquitin-protein ligase AMFR ELISA Kit; AMFR/E3 ubiquitin-protein ligase AMFR/gp78/Autocrine motility factor receptor/AMF receptor/RING finger protein 45/GP78/RNF45; AMFR elisa kit

• Reactivity: Human
• Sequence Length: 643

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Competitive ELISA, Coated with Antigen
• Detection Range: 0.625-40ng/ml
• Sensitivity: 0.375ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for AMFR elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-AMFR antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-AMFR antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the AMFR amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of AMFR can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 21071001
• NCBI GeneID: 267
• NCBI Accession #: NP_001135.3
• NCBI GenBank Nucleotide #: NM_001144.5
• Molecular Weight: 72,996 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase AMFR
• NCBI Official Synonym Full Names: autocrine motility factor receptor, E3 ubiquitin protein ligase
• NCBI Official Symbol: AMFR
• NCBI Official Synonym Symbols: GP78; RNF45
• NCBI Protein Information: E3 ubiquitin-protein ligase AMFR
• UniProt Protein Name: E3 ubiquitin-protein ligase AMFR
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: AMFR
• UniProt Synonym Gene Names: RNF45; AMF receptor
• UniProt Entry Name: AMFR_HUMAN

NCBI Description

This locus encodes a glycosylated transmembrane receptor. Its ligand, autocrine motility factor, is a tumor motility-stimulating protein secreted by tumor cells. The encoded receptor is also a member of the E3 ubiquitin ligase family of proteins. It catalyzes ubiquitination and endoplasmic reticulum-associated degradation of specific proteins. [provided by RefSeq, Feb 2012]

Uniprot Description

AMFR: E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97- AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97- AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis. Interacts with RNF5. Also forms an ERAD complex containing VCP/p97, NGLY1; PSMC1; SAKS1 AND RAD23B required for coupling retrotranslocation, ubiquitination and deglycosylation. Interacts with DRL1. Interacts (through a region distinct from the RING finger) with UBE2G2/UBC7. Component of the VCP/p97-AMFR/gp78 complex that enhances VCP/p97 binding to polyubiquitinated proteins for their degradation by the endoplasmic reticulum-associated degradation (ERAD) pathway. Interacts (via the VIM) with VCP/p97. Interacts (via its membrane domain) with INSIG1; the interaction initiates the sterol-mediated ubiquitination and degradation of HMGCR by the ERAD pathway. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Endoplasmic reticulum; Membrane protein, integral; EC 6.3.2.-; Receptor, misc.; EC 6.3.2.19; Ligase; Membrane protein, multi-pass; Motility/polarity/chemotaxis; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: cell soma; dendrite; endoplasmic reticulum membrane; growth cone; integral to endoplasmic reticulum membrane; integral to membrane; membrane; nucleus; perinuclear region of cytoplasm; protein complex

Molecular Function: chaperone binding; ligase activity; protein binding; protein binding, bridging; receptor activity; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: aging; cell motility; ER-associated protein catabolic process; learning and/or memory; positive regulation of protein binding; protein autoubiquitination; protein oligomerization; protein polyubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; signal transduction; ubiquitin-dependent protein catabolic process; unfolded protein response