Goat DGAT1 Polyclonal Antibody | anti-DGAT1 antibody

DGAT1 (Diacylglycerol O-acyltransferase 1, ACAT-related Gene Product 1, Diglyceride Acyltransferase, AGRP1, DGAT)

Cat. Num. AAA646900

• Gene Names: DGAT1; ARAT; DGAT; ARGP1
• Reactivity: Canine, Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: DGAT1; Polyclonal Antibody; DGAT1 (Diacylglycerol O-acyltransferase 1; ACAT-related Gene Product 1; Diglyceride Acyltransferase; AGRP1; DGAT); Anti -DGAT1 (Diacylglycerol O-acyltransferase 1; anti-DGAT1 antibody

• Host: Goat
• Reactivity: Canine, Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human DGAT1. Species Crossreactivity
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

• Applicable Applications for anti-DGAT1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:64,000; Western Blot: 0.3-1ug/ml, observed in human and rat duodenum lysates on ~55kD bands
• Immunogen: Synthetic peptide corresponding to C-QNSMKPFKDMDYS, from DGAT1. at the internal region of the protein (NP_036211.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

((0.3ug/ml) staining of Rat Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-DGAT1 antibody

Glucose and insulin are anabolic signals which upregulate the transcriptions of a series of lipogenic enzymes to convert excess carbohydrate into triglycerides for efficient energy storage. DGAT1 is a microsomal enzyme that assists in the synthesis of fatty acids into triglycerides. DGAT catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol (DAG) and fatty acyl CoA as substrates. DGAT plays a fundamental role in the metabolism of cellular diacylglycerol and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism such as intestinal fat absorption, lipoprotein assembly, adipose tissue formation, and lactation. DGAT1 is involved in fat absorption in the intestine and in basal level triglyceride synthesis in adipose tissue, where it is more highly expressed.

Product Categories/Family for anti-DGAT1 antibody

Antibodies; Abs to Enzymes; Acyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 145864459
• NCBI GeneID: 8694
• NCBI Accession #: NP_036211.2
• NCBI GenBank Nucleotide #: NM_012079.5
• UniProt Accession #: O75907; Q96BB8; B2RWQ2; D3DWL6
• Molecular Weight: 55,278 Da
• NCBI Official Full Name: diacylglycerol O-acyltransferase 1
• NCBI Official Synonym Full Names: diacylglycerol O-acyltransferase 1
• NCBI Official Symbol: DGAT1
• NCBI Official Synonym Symbols: ARAT; DGAT; ARGP1
• NCBI Protein Information: diacylglycerol O-acyltransferase 1; ACAT related gene product 1; diglyceride acyltransferase; retinol O-fatty-acyltransferase; acyl-CoA:diacylglycerol acyltransferase; acyl-CoA retinol O-fatty-acyltransferase; acyl coenzyme A:cholesterol acyltransferase related gene 1
• UniProt Protein Name: Diacylglycerol O-acyltransferase 1
• Protein Family: Diacylglycerol O-acyltransferase
• UniProt Gene Name: DGAT1
• UniProt Synonym Gene Names: AGRP1; DGAT; ARAT; Retinol O-fatty-acyltransferase
• UniProt Entry Name: DGAT1_HUMAN

NCBI Description

This gene encodes an multipass transmembrane protein that functions as a key metabolic enzyme. The encoded protein catalyzes the conversion of diacylglycerol and fatty acyl CoA to triacylglycerol. This enzyme can also transfer acyl CoA to retinol. Activity of this protein may be associated with obesity and other metabolic diseases. [provided by RefSeq, Jul 2013]

Uniprot Description

Function: Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. In liver, plays a role in esterifying exogenous fatty acids to glycerol. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders.

Catalytic activity: Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol. Ref.5Acyl-CoA + retinol = CoA + retinyl ester. Ref.5

Enzyme regulation: XP620 is a selective DGAT1 inhibitor. Ref.5

Pathway: Lipid metabolism; glycerolipid metabolism.

Subunit structure: Homodimer or homotetramer

By similarity.

Subcellular location: Endoplasmic reticulum membrane; Multi-pass membrane protein

By similarity.

Sequence similarities: Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

Biophysicochemical propertiesKinetic parameters:KM=25.9 µM for retinol Ref.5KM=13.9 µM for palmitoyl coenzyme A