Mouse DGAT1 (Diacylglycerol O-acyltransferase 1) ELISA Kit | DGAT1 elisa kit

Mouse DGAT1 (Diacylglycerol O-acyltransferase 1)

Cat. Num. AAA766172

• Gene Names: DGAT1; ARAT; DGAT; ARGP1; DIAR7
• Reactivity: Mouse
• Synonyms: DGAT1 (Diacylglycerol O-acyltransferase 1); Mouse DGAT1 (Diacylglycerol O-acyltransferase 1); hCG_24006/ARGP1/DGAT/ACAT related gene product 1/ acyl coenzyme A:cholesterol acyltransferase related gene 1/diacylglycerol O-acyltransferase 1/diglyceride acyltransferase/diacylglycerol O-acyltransferase homolog 1; DGAT1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of DGAT1. No significant cross-reactivity or interference between DGAT1 and analogues was observed.
• Sequence Length: 488

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 18.75-1200pg/ml
• Sensitivity: 11.2pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DGAT1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DGAT1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: 4 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DGAT1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- DGAT1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- DGAT1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the DGAT1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of DGAT1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 145864459
• NCBI GeneID: 8694
• NCBI Accession #: NP_036211.2
• NCBI GenBank Nucleotide #: NM_012079.5
• Molecular Weight: 55,278 Da
• NCBI Official Full Name: diacylglycerol O-acyltransferase 1
• NCBI Official Synonym Full Names: diacylglycerol O-acyltransferase 1
• NCBI Official Symbol: DGAT1
• NCBI Official Synonym Symbols: ARAT; DGAT; ARGP1; DIAR7
• NCBI Protein Information: diacylglycerol O-acyltransferase 1
• UniProt Protein Name: Diacylglycerol O-acyltransferase 1
• Protein Family: Diacylglycerol O-acyltransferase
• UniProt Gene Name: DGAT1
• UniProt Synonym Gene Names: AGRP1; DGAT; ARAT; Retinol O-fatty-acyltransferase
• UniProt Entry Name: DGAT1_HUMAN

NCBI Description

This gene encodes an multipass transmembrane protein that functions as a key metabolic enzyme. The encoded protein catalyzes the conversion of diacylglycerol and fatty acyl CoA to triacylglycerol. This enzyme can also transfer acyl CoA to retinol. Activity of this protein may be associated with obesity and other metabolic diseases. [provided by RefSeq, Jul 2013]

Uniprot Description

DGAT1: Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. In liver, plays a role in esterifying exogenous fatty acids to glycerol. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders. Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

Protein type: Transferase; EC 2.3.1.76; Cofactor and Vitamin Metabolism - retinol; EC 2.3.1.20; Membrane protein, multi-pass; Membrane protein, integral; Lipid Metabolism - glycerolipid

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: 2-acylglycerol O-acyltransferase activity; diacylglycerol O-acyltransferase activity; protein binding; retinol O-fatty-acyltransferase activity; transferase activity, transferring acyl groups

Biological Process: diacylglycerol metabolic process; fatty acid homeostasis; retinol metabolic process; sequestering of lipid; triacylglycerol biosynthetic process; triacylglycerol metabolic process

Disease: Diarrhea 7