Rabbit anti-Human DEN5A Polyclonal Antibody | anti-DEN5A antibody

DEN5A, ID (DENND5A, KIAA1091, RAB6IP1, DENN domain-containing protein 5A, Rab6-interacting protein 1) (FITC)

Cat. Num. AAA6291698

• Gene Names: DENND5A; RAB6IP1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DEN5A; Polyclonal Antibody; ID (DENND5A; KIAA1091; RAB6IP1; DENN domain-containing protein 5A; Rab6-interacting protein 1) (FITC); DENND5A; Rab6-interacting protein 1; anti-DEN5A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-DEN5A antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DEN5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 702-732 amino acids from the Central region of human DEN5A.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DEN5A Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the DEN5A antibody detected the DEN5A protein (arrow).)

Flow Cytometry (FC/FACS)

(DEN5A Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-DEN5A antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Recacha, R., et al. Structure 17(1):21-30(2009). Olsen, J.V., et al. Cell 127(3):635-648(2006). Janoueix-Lerosey, I., et al. J. Biol. Chem. 270(24):14801-14808(1995).

NCBI and Uniprot Product Information

• NCBI GI #: 44889475
• NCBI GeneID: 23258
• NCBI Accession #: NP_056028.2
• NCBI GenBank Nucleotide #: NM_015213.3
• UniProt Accession #: Q6IQ26; Q96GN3; Q9H6U7; Q9UFV0; Q9UPR1; B4DJ15; E9PS91
• Molecular Weight: 141,982 Da
• NCBI Official Full Name: DENN domain-containing protein 5A isoform 1
• NCBI Official Synonym Full Names: DENN domain containing 5A
• NCBI Official Symbol: DENND5A
• NCBI Official Synonym Symbols: RAB6IP1
• NCBI Protein Information: DENN domain-containing protein 5A
• UniProt Protein Name: DENN domain-containing protein 5A
• UniProt Gene Name: DENND5A
• UniProt Synonym Gene Names: KIAA1091; RAB6IP1; Rab6IP1
• UniProt Entry Name: DEN5A_HUMAN

Uniprot Description

RAB6IP1: a Golgi-associated guanine nucleotide exchange factor (GEF) that activates RAB6A and RAB39A/B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Interacts with RAB6A bound to GTP. Belongs to the RAB6IP1 family.

Protein type: GEFs, Rab; GEFs

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: membrane; retromer complex; trans-Golgi network

Molecular Function: calcium channel activity; Rab guanyl-nucleotide exchange factor activity; Rab GTPase binding

Biological Process: detection of mechanical stimulus; retrograde transport, endosome to Golgi