Rabbit anti-Human PEDF Polyclonal Antibody | anti-alpha-PEDF antibody

anti-Human PEDF Antibody (polyclonal, rabbit)

Cat. Num. AAA412002

• Gene Names: SERPINF1; OI6; OI12; PEDF; EPC-1; PIG35
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: alpha-PEDF Antibody has been shown to be >90% pure by SDS-PAGE. alpha-PEDF Antibody is provided as a non-sterile sample. The product may be rendered sterile by 0.22 um filtration after reconstitution.
• Synonyms: PEDF; Polyclonal Antibody; anti-Human PEDF Antibody (polyclonal; rabbit); RABBIT POLYCLONAL AFFINITY PUR.; anti-alpha-PEDF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: alpha-PEDF Antibody reacts specifically with PEDF by Western Blotting. Recommended dilution range for Western analysis: 1:1,000 - 1:10,000. Recommended starting dilution: 1:5,000.
• Purity/Purification: alpha-PEDF Antibody has been shown to be >90% pure by SDS-PAGE. alpha-PEDF Antibody is provided as a non-sterile sample. The product may be rendered sterile by 0.22 um filtration after reconstitution.
• Concentration: 1.0 mg/mL after reconstitution. (varies by lot)
• Sequence Length: 418

• Applicable Applications for anti-alpha-PEDF antibody: Western Blot (WB), ELISA (EIA)
• Immunogen: human recombinant PEDF
• Source Notes: alpha-PEDF Antibody is an affinity purified rabbit polyclonal antibody raised against purified human PEDF protein.
• Reconstitution: Reconstitute lyophilized alpha-PEDF Antibody in 100 uL diH2O.
• Preparation and Storage: alpha-PEDF Antibody is shipped at ambient temperature. This product is stable for at least 1 year following receipt. Store at 4 degree C. Do Not Freeze!

Testing Data

Related Product Information for anti-alpha-PEDF antibody

Background: Pigment epithelium-derived factor (PEDF) is a protein that acts in neuronal differentiation and survival in cells derived from the retina and CNS. PEDF inhibits angiogenesis and its expression is down-regulated over the replicative lifespan of mammals. This interesting factor is secreted by retinal pigment epithelial cells into the interphotoreceptor matrix, where it acts on photoreceptor cells. PEDF receptors have been localized to photoreceptors, those cells that are protected from light-induced damage and apoptosis. PEDF promotes neuronal survival through activation of NF?B, which in turn induces expression of anti-apoptotic and/or neurotrophic factor genes. Its importance in the development, maintenance, and function of the retina and CNS is evident in animal models for inherited and light induced retinal degeneration, as well as for degeneration of spinal cord motor neurons, and animal models for diseases triggered by choroidal and retinal neovascularization. PEDF is a member of the serpin superfamily of protease inhibitors, but it has characteristics of a substrate rather than an inhibitor of serine proteases. An N-terminus peptide region provides the neurotrophic function to the PEDF protein while other structural characteristics are dispensable (e.g. signal peptide, oligosaccharides on the polypeptide backbone, serpin exposed loop).

References

Becerra SP. (1997) Structure-function studies on PEDF. A non-inhibitory serpin with neurotrophic activity. Adv Exp Med Biol, 425, 223-237.
Cao, W, Tombran-Tink, J, Elias, R, Sezate,S, Mrazed, D, McGinnis, JF. (2001) In vivo protection of photoreceptors from light damage by pigment epithelium-derived factor. Invest Ophthalmol Vis Sci, 42, 1646-1652.
Cayouette M, Smith SB, Becerra SP, Gravel C. (1999) Pigment epithelium-derived factor delays the death of photoreceptors in mouse models of inherited retinal degenerations. Neurobiol Dis, Dec;6(6), 523-532.
Dawson DW, Volpert OV, Gillis P, Crawford SE, Xu H, Benedict W, Bouck NP. (1999) Pigment epithelium-derived factor: a potent inhibitor of angiogenesis. Science, 285(5425), 245-248.
Houenou, LJ, D'Costa, AP, Li, L, Turgeon, VL, Enyadike, C, Alberdi, E, and Becerra, SP. (1999) Pigment epithelium-derived factor promotes the survival and differentiation of developing spinal motor neurons. J. Comp Neurol, 412, 506-514.
Jablonski, MM, Tombran-Tink, J, Mrazed, DA, and Iannaccone, A. (2000) Pigment epithelium derived factor supports normal development of photoreceptor neurons and opsin expression after retinal pigment epithelium removal.J. Neurosci, 20, 7149-7157.
Mori, K, Gehlbach, P, Ando, A, McVey, D, Wei, L, and Campochiaro, PA. (2002) Regression of ocular neovascularization in response to increased expression of pigment epithelium-derived factor. Invest Ophthalmol Vis Sci, 43, 2428-2434.
Stellmach, V, Crawford, SE, Zhou, W, and Bouck, N. (2001) Prevention of ischemia-induced retinopathy by the natural ocular antiangiogenic agent pigment epithelium-derived factor. ProcNatl Acad Sci USA, 98, 2593-2597.

NCBI and Uniprot Product Information

• NCBI GI #: 39725934
• NCBI GeneID: 5176
• NCBI Accession #: NP_002606.3
• NCBI GenBank Nucleotide #: NM_002615.5
• NCBI Official Full Name: pigment epithelium-derived factor
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
• NCBI Official Symbol: SERPINF1
• NCBI Official Synonym Symbols: OI6; OI12; PEDF; EPC-1; PIG35
• NCBI Protein Information: pigment epithelium-derived factor; serpin F1; cell proliferation-inducing gene 35 protein; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1

NCBI Description

The protein encoded by this gene is a member of the serpin family, although it does not display the serine protease inhibitory activity shown by many of the other serpin family members. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells.[provided by RefSeq, Mar 2011]

Uniprot Description

PEDF: a secreted neurotrophic protein that induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. Extracellular phosphorylation enhances antiangiogenic activity.

Protein type: Secreted; Secreted, signal peptide; Inhibitor

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: axon hillock; extracellular matrix; extracellular space; perinuclear region of cytoplasm; melanosome; extracellular region; basement membrane

Molecular Function: serine-type endopeptidase inhibitor activity

Biological Process: cell proliferation; negative regulation of angiogenesis; positive regulation of neurogenesis; ovulation cycle; short-term memory; retina development in camera-type eye; multicellular organismal development; negative regulation of inflammatory response; response to arsenic; response to acidity; kidney development; aging

Disease: Osteogenesis Imperfecta, Type Vi