Rabbit DEK Polyclonal Antibody | anti-DEK antibody

Anti-DEK Antibody, Rabbit Polyclonal

Cat. Num. AAA8101055

• Gene Names: DEK; D6S231E
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: DEK; Polyclonal Antibody; Anti-DEK Antibody; Rabbit Polyclonal; DEK Antibody; Rabbit PAb; Antigen Affinity Purified; DEK proto-oncogene; Anti-D6S231E Antibody; anti-DEK antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human DEK
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-DEK antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; ICC/IF: 1:1500-1:50,000; IP: 0.2-1uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the center region of the Human DEK
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human DEK, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-DEK rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:50 kDa)

Immunofluorescence (IF)

(Immunofluorescence staining of DEK in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human DEK polyclonal antibody (dilution ratio 1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.)

Immunoprecipitation (IP)

(DEK was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate 1 uL anti-DEK rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-DEK rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:500 dilution Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 42 kDa Observed band size: 42 kDa)

Related Product Information for anti-DEK antibody

The DNA binding protein and chromatin structural regulator DEK regulate many cellular processes. These include proliferation, differentiation, apoptosis, senescence, DNA repairing and the maintenance of stem cell phenotype. DEK is increasingly recognized as a crucial player in many steps of cancer initiation and progression, and is precisely regulated by abundant promoting and inhibiting factors directly or indirectly. DEK may serve as an architectural modulating protein to regulate the expression and function of multiple human genes in cancer cells. DEK is a highly conserved chromatin-bound protein whose upregulation across cancer types correlates with genotoxic therapy resistance. Loss of DEK induces genome instability and sensitizes cells to DNA double strand breaks (DSBs), suggesting defects in DNA repair. The DEK oncogene is overexpressed in many human malignancies including at early tumor stages. The high levels of DEK significantly reprogrammed cellular metabolism and altered the abundances of amino acids, TCA cycle intermediates and the glycolytic end products lactate, alanine and NAD+. Decreased DEK expression in plasma cells suggests a potential role of this gene in plasma cell development and lack of detectable DEK protein by IHC could be used as a biomarker for normal and malignant plasma cells.

NCBI and Uniprot Product Information

• NCBI GI #: 544150
• NCBI GeneID: 7913
• UniProt Accession #: P35659; Q5TGV4; Q5TGV5; B2R6K6; B4DN37
• Molecular Weight: Predicted: 43 kDa
• NCBI Official Full Name: Protein DEK
• NCBI Official Synonym Full Names: DEK proto-oncogene
• NCBI Official Symbol: DEK
• NCBI Official Synonym Symbols: D6S231E
• NCBI Protein Information: protein DEK
• UniProt Protein Name: Protein DEK
• Protein Family: Protein
• UniProt Gene Name: DEK
• UniProt Entry Name: DEK_HUMAN

NCBI Description

This gene encodes a protein with one SAP domain. This protein binds to cruciform and superhelical DNA and induces positive supercoils into closed circular DNA, and is also involved in splice site selection during mRNA processing. Chromosomal aberrations involving this region, increased expression of this gene, and the presence of antibodies against this protein are all associated with various diseases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

DEK: a ubiquitous protein with a SAP domain, which is found in a variety of nuclear proteins involved in transcription, DNA repair, RNA processing or apoptotic chromatin degradation. This protein binds to cruciform and superhelical DNA and induces positive supercoils into closed circular DNA and is also involved in splice site selection during mRNA processing. A chromosomal aberration involving DEK is found in a subset of acute myeloid leukemias (AML).

Protein type: Histone-binding; Oncoprotein; DNA-binding; DNA replication; RNA splicing

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: contractile fiber; nucleoplasm; nucleus

Molecular Function: DNA binding; histone binding

Biological Process: chromatin modification; positive regulation of gene expression, epigenetic; regulation of transcription from RNA polymerase II promoter; signal transduction; transcription from RNA polymerase II promoter; viral genome replication