Rabbit anti-Human, Mouse DDC Polyclonal Antibody | anti-DDC antibody

DDC (Aromatic-L-amino-acid Decarboxylase, AADC, DOPA Decarboxylase, DDC) (HRP)

Cat. Num. AAA6375824

• Gene Names: DDC; AADC
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DDC; Polyclonal Antibody; DDC (Aromatic-L-amino-acid Decarboxylase; AADC; DOPA Decarboxylase; DDC) (HRP); anti-DDC antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DDC. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-DDC antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human DDC, aa1-480 (NP_000781.1).
• Immunogen Sequence: MNASEFRRRGKEMVDYVANYMEGIEGRQVYPDVEPGYLRPLIPAAAPQEPDTFEDIINDVEKIIMPGVTHWHSPYFFAYFPTASSYPAMLADMLCGAIGCIGFSWAASPACTELETVMMDWLGKMLELPKAFLNEKAGEGGGVIQGSASEATLVALLAARTKVIHRLQAASPELTQAAIMEKLVAYSSDQAHSSVERAGLIGGVKLKAIPSDGNFAMRASALQEALERDKAAGLIPFFMVATLGTTTCCSFDNLLEVGPICNKEDIWLHVDAAYAGSAFICPEFRHLLNGVEFADSFNFNPHKWLLVNFDCSAMWVKKRTDLTGAFRLDPTYLKHSHQDSGLITDYRHWQIPLGRRFRSLKMWFVFRMYGVKGLQAYIRKHVQLSHEFESLVRQDPRFEICVEVILGLVCFRLKGSNKVNEALLQRINSAKKIHLVPCHLRDKFVLRFAICSRTVESAHVQRAWEHIKELAADVLRAERE
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of DDC expression in transfected 293T cell line by DDC polyclonal antibody. Lane 1: DDC transfected lysate (53.9kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-DDC antibody

DOPA decarboxylase (aromatic L-amino acid decarboxylase AADC, DDC) catalyzes the second reaction in the biosynthesis of catecholamines and serotonin. It is also involved in the biosynthesis of trace amines.

Product Categories/Family for anti-DDC antibody

Antibodies; Enzymes; Decarboxylase

NCBI and Uniprot Product Information

• NCBI GI #: 4503281
• NCBI GeneID: 1644
• NCBI Accession #: NP_000781.1
• NCBI GenBank Nucleotide #: NM_000790.2
• UniProt Accession #: P20711; Q16723; Q5W5T9; Q75MJ6; C9IYA0; E7ER62; E7EU95
• Molecular Weight: 44,239 Da
• NCBI Official Full Name: aromatic-L-amino-acid decarboxylase isoform 1
• NCBI Official Synonym Full Names: dopa decarboxylase (aromatic L-amino acid decarboxylase)
• NCBI Official Symbol: DDC
• NCBI Official Synonym Symbols: AADC
• NCBI Protein Information: aromatic-L-amino-acid decarboxylase
• UniProt Protein Name: Aromatic-L-amino-acid decarboxylase
• Protein Family: Aromatic-L-amino-acid decarboxylase
• UniProt Gene Name: DDC
• UniProt Synonym Gene Names: AADC; AADC; DDC
• UniProt Entry Name: DDC_HUMAN

NCBI Description

The encoded protein catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Defects in this gene are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2011]

Uniprot Description

DDC: Catalyzes the decarboxylation of L-3,4- dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Homodimer. Belongs to the group II decarboxylase family.

Protein type: Amino Acid Metabolism - tryptophan; EC 4.1.1.28; Amino Acid Metabolism - histidine; Amino Acid Metabolism - tyrosine; Nuclear receptor co-regulator; Amino Acid Metabolism - phenylalanine; Lyase

Chromosomal Location of Human Ortholog: 7p12.2

Cellular Component: synaptic vesicle; cell soma; axon; cytosol

Molecular Function: aromatic-L-amino-acid decarboxylase activity; protein domain specific binding; amino acid binding; protein binding; enzyme binding; pyridoxal phosphate binding

Biological Process: circadian rhythm; amino acid metabolic process; catecholamine biosynthetic process; indolalkylamine biosynthetic process; multicellular organismal aging; isoquinoline alkaloid metabolic process; dopamine biosynthetic process; serotonin biosynthetic process; phytoalexin metabolic process; synaptic vesicle amine transport; response to pyrethroid

Disease: Aromatic L-amino Acid Decarboxylase Deficiency