Human CKMB ELISA Kit | CKMB elisa kit

Human CKMB(Creatine Kinase MB Isoenzyme)ELISA Kit

Cat. Num. AAA2507456

• Gene Names: Ckm; Ckmm
• Reactivity: Human
• Synonyms: CKMB; Human CKMB(Creatine Kinase MB Isoenzyme)ELISA Kit; CKMB elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human CKMB. No significant cross-reactivity or interference between Human CKMB and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human CKMB and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 381

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 7.813-500ng/mL
• Sensitivity: Min: 4.688ng/mL; Max: 500ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CKMB elisa kit

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CKMB in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CKMB. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human CKMB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CKMB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CKMB. You can calculate the concentration of Human CKMB in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6978661
• NCBI GeneID: 24265
• NCBI Accession #: NP_036662.1
• NCBI GenBank Nucleotide #: NM_012530.2
• UniProt Accession #: P00564; Q6P6R9
• Molecular Weight: 43,045 Da
• NCBI Official Full Name: creatine kinase M-type
• NCBI Official Synonym Full Names: creatine kinase, muscle
• NCBI Official Symbol: Ckm
• NCBI Official Synonym Symbols: Ckmm
• NCBI Protein Information: creatine kinase M-type; M-CK; creatine kinase M chain
• UniProt Protein Name: Creatine kinase M-type
• UniProt Gene Name: Ckm
• UniProt Synonym Gene Names: Ckmm
• UniProt Entry Name: KCRM_RAT

NCBI Description

catalyzes regeneration of ATP; expression is induced by p53 [RGD, Feb 2006]

Uniprot Description

CKM: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.

Protein type: Amino Acid Metabolism - arginine and proline; Kinase, other; EC 2.7.3.2

Cellular Component: cytoplasm

Molecular Function: creatine kinase activity; ATP binding

Biological Process: phosphocreatine biosynthetic process; phosphorylation