Rabbit DCK / Deoxycytidine kinase Polyclonal Antibody | anti-DCK antibody

Anti-DCK / Deoxycytidine kinase Antibody (aa1-260) IHC-plus

Cat. Num. AAA2400035

• Reactivity: Mouse, Rat, Human
• Applications: Immunohistochemistry, Immunocytochemistry, Western Blot
• Purity: Immunoaffinity purified
• Synonyms: DCK / Deoxycytidine kinase; Polyclonal Antibody; Anti-DCK / Deoxycytidine kinase Antibody (aa1-260) IHC-plus; Rabbit Polyclonal to Human DCK / Deoxycytidine kinase; Human DCK / Deoxycytidine kinase; Deoxynucleoside kinase; Deoxycytidine kinase; anti-DCK antibody

• Host: Rabbit
• Reactivity: Mouse, Rat, Human
• Clonality: Polyclonal
• Specificity: Human DCK
• Purity/Purification: Immunoaffinity purified
• Form/Format: 0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 260

• Applicable Applications for anti-DCK antibody: Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Western Blot (WB)
• Application Notes: ICC (1:100 - 1:1000); IHC-P (7.5 ug/ml); WB (1:500 - 1:3000)
• Immunogen: DCK / Deoxycytidine kinase antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 260 of DCK (SwissProt P27707).
• Immunogen Description: Recombinant fragment corresponding to a region within amino acids 1 and 260 of DCK (SwissProt P27707).
• Immunogen Type: Recombinant protein
• Antigen Modification: aa1-260
• Target Species: Human
• Preparation and Storage: Keep as concentrated solution. Aliquot and store at -20 degree C or below. Avoid multiple freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-DCK / Deoxycytidine kinase antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.)

Immunocytochemistry (ICC)

(Immunofluorescence of paraformaldehyde-fixed HeLa using Deoxycytidine kinase antibody at 1:200 dilution.)

Western Blot (WB)

(Sample (30 ug whole cell lysate). A: Raji. 12% SDS PAGE. DCK antibody diluted at 1:1000)

Western Blot (WB)

(DCK antibody detects DCK protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. B. 30 ug Rat2 whole cell lysate/extract. 12 % SDS-PAGE. DCK antibody dilution:1:1000)

Western Blot (WB)

(Sample (50 ug of whole cell lysate). A: mouse brain. 12% SDS PAGE. DCK antibody diluted at 1:1000.)

Related Product Information for anti-DCK antibody

DCK Antibody, Deoxynucleoside kinase Antibody, Deoxycytidine kinase Antibody Description: Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity.

NCBI and Uniprot Product Information

• NCBI GI #: 4503269
• NCBI GeneID: 1633
• NCBI Accession #: NP_000779.1
• NCBI GenBank Nucleotide #: NM_000788.2
• UniProt Accession #: P27707; Q5TZY7; Q6FI11; B2R8V6
• Molecular Weight: 30,519 Da
• NCBI Official Full Name: deoxycytidine kinase
• NCBI Official Synonym Full Names: deoxycytidine kinase
• NCBI Official Symbol: DCK
• NCBI Protein Information: deoxycytidine kinase
• UniProt Protein Name: Deoxycytidine kinase
• Protein Family: Deoxycytidine kinase
• UniProt Gene Name: DCK
• UniProt Synonym Gene Names: dCK
• UniProt Entry Name: DCK_HUMAN

NCBI Description

Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq, Jul 2008]

Uniprot Description

DCK: Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents. Belongs to the DCK/DGK family.

Protein type: Kinase, other; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; EC 2.7.1.74

Chromosomal Location of Human Ortholog: 4q13.3-q21.1

Cellular Component: cytosol; nucleus

Molecular Function: ATP binding; deoxycytidine kinase activity; drug binding; nucleoside kinase activity; protein homodimerization activity

Biological Process: deoxyribonucleoside monophosphate biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; nucleotide biosynthetic process; phosphorylation; purine base metabolic process; purine salvage; pyrimidine base metabolic process; pyrimidine nucleoside salvage; pyrimidine nucleotide metabolic process