Mouse HSP27 Monoclonal Antibody | anti-HSP27 antibody

HSP27 (Heat Shock Protein 27, Heat shock 27kD Protein, Estrogen-regulated 24kD Protein, Heat shock 25kD Protein 1, Heat shock 28kD Protein 1, Heat shock Protein beta-1, HMN2B, Hsp25, Hsp28, HspB1, Stress-responsive Protein 27, SRP27) (FITC)

Cat. Num. AAA6124273

• Gene Names: HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067
• Reactivity: Chicken, Human, Mouse, Rat, Sheep
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: HSP27; Monoclonal Antibody; HSP27 (Heat Shock Protein 27; Heat shock 27kD Protein; Estrogen-regulated 24kD Protein; Heat shock 25kD Protein 1; Heat shock 28kD Protein 1; Heat shock Protein beta-1; HMN2B; Hsp25; Hsp28; HspB1; Stress-responsive Protein 27; SRP27) (FITC); Heat Shock Protein 27; SRP27; anti-HSP27 antibody

• Host: Mouse
• Reactivity: Chicken, Human, Mouse, Rat, Sheep
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: SPM252
• Specificity: Recognizes HSP27. Species Crossreactivity: Human, Chimpanzee, Sheep, Rat, Mouse, and Chicken.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Fluorescein isothiocyanate (FITC).

• Applicable Applications for anti-HSP27 antibody: Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: FC/FACS: 0.5-1ug/million cells; IP: 0.5-1ug/500ug protein lysate; IF: 0.5-1ug/ml; WB: 0.25-0.5ug/ml; IHC: 0.5-1ug/ml for 30 minutes at RT; Applications are based on unconjugated antibody.
• Immunogen: Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells. Cellular Localization: Predominantly cytoplasmic with some nuclear
• Conjugate: FITC
• Positive Control: ~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor.
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.; ; FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-paraffin breast carcinoma (10X) stained with HSP27 MAb (SPM252)))

Immunohistochemistry (IHC)

(Formalin-paraffin breast carcinoma (20X) stained with HSP27 MAb (SPM252))

Product Categories/Family for anti-HSP27 antibody

Antibodies; Heat Shock Proteins

References

1. Edwards DP et. al. Biochem Biophys Research Commun, 93:804-812, 1980. 2. Ciocca DR et. al. Breast Cancer Research and Treatment, 20:33-42, 1991.

NCBI and Uniprot Product Information

• NCBI GI #: 4504517
• NCBI GeneID: 3315
• NCBI Accession #: NP_001531.1
• NCBI GenBank Nucleotide #: NM_001540.3
• UniProt Accession #: P04792; P14602; P42930
• Molecular Weight: 22,783 Da
• NCBI Official Full Name: heat shock protein beta-1
• NCBI Official Synonym Full Names: heat shock 27kDa protein 1
• NCBI Official Symbol: HSPB1
• NCBI Official Synonym Symbols: CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067
• NCBI Protein Information: heat shock protein beta-1; HSP 27; 28 kDa heat shock protein; heat shock 27 kDa protein; heat shock 27kD protein 1; stress-responsive protein 27; estrogen-regulated 24 kDa protein
• UniProt Protein Name: Heat shock protein beta-1
• Protein Family: Heat shock protein
• UniProt Gene Name: HSPB1
• UniProt Synonym Gene Names: HSP27; HSP28; HspB1; HSP 27; SRP27
• UniProt Entry Name: HSPB1_HUMAN

NCBI Description

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]

Uniprot Description

HSP27: a small heat shock protein that is regulated both transcriptionally and posttranslationally. Modulates actin polymerization and reorganization. Its expression level increases several-fold in response to stress and is phosphorylated by MAPKAP kinase 2. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock.

Protein type: Chaperone; Motility/polarity/chemotaxis; Heat shock protein

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: proteasome complex; extracellular space; focal adhesion; cytoskeleton; cytoplasm; plasma membrane; spindle; cytosol; Z disc; nucleus

Molecular Function: protein kinase C inhibitor activity; identical protein binding; ubiquitin binding; protein binding; protein kinase C binding; protein kinase binding

Biological Process: positive regulation of tumor necrosis factor biosynthetic process; response to virus; positive regulation of blood vessel endothelial cell migration; response to unfolded protein; positive regulation of interleukin-1 beta production; retinal homeostasis; positive regulation of angiogenesis; negative regulation of protein kinase activity; gene expression; vascular endothelial growth factor receptor signaling pathway; cell motility; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of translational initiation; negative regulation of apoptosis

Disease: Neuronopathy, Distal Hereditary Motor, Type Iib; Charcot-marie-tooth Disease, Axonal, Type 2f