Western Blot (WB)
(Cell lysates of Jurkat (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 tau (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 tau antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant Human YWHAZ Lane 2. : Recombinant Human YWHAB Lane 3. : Recombinant Human YWHAE Lane 4. : Recombinant Human YWHAH Lane 5. : Recombinant Human YWHAG Lane 6. : Recombinant Human SFN Lane 7. : Recombinant Human YWHAQ The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 tau antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : MDA-MB-21 cell lysate Lane 2. : HeLa cell lysate Lane 3. : A431 cell lysate Lane 4. : NIH3T3 cell lysate Lane 5. : 293T cell lysate Lane 6. : HepG2 cell lysate)
Mouse anti-Human 14-3-3 tau Monoclonal Antibody | anti-14-3-3 tau antibody
14-3-3 tau antibody
Recommended starting dilution is 1:1000.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(Cell lysates of Jurkat (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 tau (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 tau antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant Human YWHAZ Lane 2. : Recombinant Human YWHAB Lane 3. : Recombinant Human YWHAE Lane 4. : Recombinant Human YWHAH Lane 5. : Recombinant Human YWHAG Lane 6. : Recombinant Human SFN Lane 7. : Recombinant Human YWHAQ The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 tau antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : MDA-MB-21 cell lysate Lane 2. : HeLa cell lysate Lane 3. : A431 cell lysate Lane 4. : NIH3T3 cell lysate Lane 5. : 293T cell lysate Lane 6. : HepG2 cell lysate)
Western Blot (WB)
(Cell lysates of Jurkat (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 tau (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 tau antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant Human YWHAZ Lane 2. : Recombinant Human YWHAB Lane 3. : Recombinant Human YWHAE Lane 4. : Recombinant Human YWHAH Lane 5. : Recombinant Human YWHAG Lane 6. : Recombinant Human SFN Lane 7. : Recombinant Human YWHAQ The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 tau antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : MDA-MB-21 cell lysate Lane 2. : HeLa cell lysate Lane 3. : A431 cell lysate Lane 4. : NIH3T3 cell lysate Lane 5. : 293T cell lysate Lane 6. : HepG2 cell lysate)
Immunofluorescence (IF)
(ICC/IF analysis of 14-3-3 tau in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 tau antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(ICC/IF analysis of 14-3-3 tau in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 tau antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
NCBI and Uniprot Product Information
NCBI Description
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008]
Uniprot Description
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
Research Articles on 14-3-3 tau
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Product Notes
The 14-3-3 tau ywhaq (Catalog #AAA200131) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The 14-3-3 tau antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's 14-3-3 tau can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB: 1:1000 ~ 2000. Recommended starting dilution is 1:1000. Researchers should empirically determine the suitability of the 14-3-3 tau ywhaq for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "14-3-3 tau, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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