Rabbit anti-Human DC8 Polyclonal Antibody | anti-DC8 antibody

DC8 antibody

Cat. Num. AAA9405443

• Gene Names: NSL1; DC8; MIS14; C1orf48
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Purified by antigen-affinity chromatography.
• Synonyms: DC8; Polyclonal Antibody; DC8 antibody; anti-DC8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified by antigen-affinity chromatography.
• Form/Format: Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Sequence Length: 281

• Applicable Applications for anti-DC8 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500-1:3000; Immunofluorescence: 1:100-1:200
• Immunogen Type: Recombinant protein
• Immunogen Description: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 245 of DC8
• Target Name: DC8
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Testing Data

(Sample(30 ug of whole cell lysate)A: Hep G212% SDS PAGEPrimary antibody diluted at 1: 1000)

Immunofluorescence (IF)

(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DC8 antibody at 1: 200 dilution.)

Related Product Information for anti-DC8 antibody

This gene encodes a protein with two coiled-coil domains that localizes to kinetochores, which are chromosome-associated structures that attach to microtubules and mediate chromosome movements during cell division. The encoded protein is part of a conserved protein complex that includes two chromodomain-containing proteins and a component of the outer plate of the kinetochore. This protein complex is proposed to bridge centromeric heterochromatin with the outer kinetochore structure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Product Categories/Family for anti-DC8 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 110349759
• NCBI GeneID: 25936
• NCBI Accession #: NP_056286
• NCBI GenBank Nucleotide #: NM_015471.3
• Molecular Weight: 24,108 Da
• NCBI Official Full Name: kinetochore-associated protein NSL1 homolog isoform 1
• NCBI Official Synonym Full Names: NSL1, MIS12 kinetochore complex component
• NCBI Official Symbol: NSL1
• NCBI Official Synonym Symbols: DC8; MIS14; C1orf48
• NCBI Protein Information: kinetochore-associated protein NSL1 homolog
• UniProt Protein Name: Kinetochore-associated protein NSL1 homolog
• UniProt Gene Name: NSL1
• UniProt Synonym Gene Names: C1orf48
• UniProt Entry Name: NSL1_HUMAN

NCBI Description

This gene encodes a protein with two coiled-coil domains that localizes to kinetochores, which are chromosome-associated structures that attach to microtubules and mediate chromosome movements during cell division. The encoded protein is part of a conserved protein complex that includes two chromodomain-containing proteins and a component of the outer plate of the kinetochore. This protein complex is proposed to bridge centromeric heterochromatin with the outer kinetochore structure. Multiple transcript variants encoding different isoforms have been found for this gene. There is a pseudogene of the 3' UTR region of this gene on chromosome X. [provided by RefSeq, Jul 2014]

Uniprot Description

NSL1: Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: nucleus; cytosol; MIS12/MIND type complex

Molecular Function: protein binding

Biological Process: mitosis; cell division; mitotic cell cycle; chromosome segregation