Rabbit anti-Human NSL1 Polyclonal Antibody | anti-NSL1 antibody

Anti-NSL1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103676

• Gene Names: NSL1; DC8; MIS14; C1orf48
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: NSL1; Polyclonal Antibody; Anti-NSL1 Antibody; Rabbit Polyclonal; NSL1 Antibody; Rabbit PAb; Antigen Affinity Purified; MIS12 kinetochore complex component; Anti-C1orf48 Antibody; Anti-DC8 Antibody; Anti-MIS14 Antibody; anti-NSL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human NSL1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-NSL1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; ICC/IF: 1:1500-1:50,000
• Immunogen: A synthetic peptide corresponding to the center region of the Human NSL1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human NSL1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-NSL1 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:32 kDa Observed band size:34 kDa)

Immunofluorescence (IF)

(Immunofluorescence staining of NSL1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human NSL1 polyclonal antibody (dilution ratio 1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)

Related Product Information for anti-NSL1 antibody

NSL1 (NSL1 Component Of MIS12 Kinetochore Complex) is a Protein Coding gene. The NSL1 gene, located on 1q32.3, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, and frog. This gene encodes a protein with two coiled-coil domains that localize to kinetochores, which are chromosome-associated structures that attach to microtubules and mediate chromosome movements during cell division. The encoded protein is part of a conserved protein complex that includes two chromodomain-containing proteins and a component of the outer plate of the kinetochore. Multiple transcript variants encoding different isoforms have been found for this gene. There is a pseudogene of the 3' UTR region of this gene on chromosome X. Diseases associated with NSL1 include Arthrogryposis, Distal, Type 7, and Extraocular Retinoblastoma. Among its related pathways are Signaling by GPCR and Mitotic Prometaphase.

References

Petrovic A, et al. (2010) The mis12 complex is a protein interaction hub for outer kinetochore assembly. J Cell Biol 190 (5): 835-852.Liu XS, et al. (2012) Plk1 phosphorylates sgt1 at the kinetochores to promote timely kinetochore-microtubule attachment. Mol Cell Biol 32 (19): 4053-4067.Davies AE, et al. (2010) Hsp90-sgt1 and skp1 target human mis12 complexes to ensure efficient formation of kinetochore-microtubule binding sites. J Cell Biol 189 (2): 261-274.

NCBI and Uniprot Product Information

• NCBI GI #: 110349761
• NCBI GeneID: 25936
• NCBI Accession #: NP_001036014.1
• NCBI GenBank Nucleotide #: NM_001042549.1
• UniProt Accession #: Q96IY1; Q5SY75; Q9H2M5; Q9NRN8; Q9Y415; E7ETD5
• Molecular Weight: 24,108 Da
• NCBI Official Full Name: kinetochore-associated protein NSL1 homolog isoform 2
• NCBI Official Synonym Full Names: NSL1, MIS12 kinetochore complex component
• NCBI Official Symbol: NSL1
• NCBI Official Synonym Symbols: DC8; MIS14; C1orf48
• NCBI Protein Information: kinetochore-associated protein NSL1 homolog; NSL1, MIND kinetochore complex component, homolog
• UniProt Protein Name: Kinetochore-associated protein NSL1 homolog
• Protein Family: Kinetochore-associated protein
• UniProt Gene Name: NSL1
• UniProt Synonym Gene Names: C1orf48
• UniProt Entry Name: NSL1_HUMAN

NCBI Description

This gene encodes a protein with two coiled-coil domains that localizes to kinetochores, which are chromosome-associated structures that attach to microtubules and mediate chromosome movements during cell division. The encoded protein is part of a conserved protein complex that includes two chromodomain-containing proteins and a component of the outer plate of the kinetochore. This protein complex is proposed to bridge centromeric heterochromatin with the outer kinetochore structure. Multiple transcript variants encoding different isoforms have been found for this gene. There is a pseudogene of the 3' UTR region of this gene on chromosome X. [provided by RefSeq, Jul 2014]

Uniprot Description

NSL1: Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: nucleus; cytosol; MIS12/MIND type complex

Molecular Function: protein binding

Biological Process: mitosis; cell division; mitotic cell cycle; chromosome segregation