Western Blot (WB)
(Western blot analysis of DC13 Antibody (N-term) in mouse kidney tissue lysates (35ug/lane). DC13 (arrow) was detected using the purified Pab.)
Rabbit anti-Human, Mouse DC13 Polyclonal Antibody | anti-DC13 antibody
DC13, NT (CMC2, C16orf61, COX assembly mitochondrial protein 2 homolog) (MaxLight 490)
Gene Names
CMC2; DC13; C16orf61; 2310061C15Rik
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Synonyms
DC13; Polyclonal Antibody; NT (CMC2; C16orf61; COX assembly mitochondrial protein 2 homolog) (MaxLight 490); CMC2; COX assembly mitochondrial protein 2 homolog; anti-DC13 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Applicable Applications for anti-DC13 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
DC13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 1-30 from the N-terminal region of human DC13.
Conjugate
MaxLight490
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of DC13 Antibody (N-term) in mouse kidney tissue lysates (35ug/lane). DC13 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of DC13 Antibody (N-term) in mouse kidney tissue lysates (35ug/lane). DC13 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(DC13 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DC13 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(DC13 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DC13 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(DC13 Antibody (N-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(DC13 Antibody (N-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-DC13 antibody
MaxLight 490 is a new Blue-Green photostable dye conjugate comparable to DyLight488, Alexa Fluor488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
Product Categories/Family for anti-DC13 antibody
References
Dastani, Z., et al. Eur. J. Hum. Genet. (2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
9,460 Da
NCBI Official Full Name
COX assembly mitochondrial protein 2 homolog
NCBI Official Synonym Full Names
C-x(9)-C motif containing 2
NCBI Official Symbol
CMC2
NCBI Official Synonym Symbols
DC13; C16orf61; 2310061C15Rik
NCBI Protein Information
COX assembly mitochondrial protein 2 homolog
UniProt Protein Name
COX assembly mitochondrial protein 2 homolog
UniProt Gene Name
CMC2
UniProt Synonym Gene Names
C16orf61; DC13
UniProt Entry Name
COXM2_HUMAN
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Product Notes
The DC13 cmc2 (Catalog #AAA6290953) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DC13, NT (CMC2, C16orf61, COX assembly mitochondrial protein 2 homolog) (MaxLight 490) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DC13 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the DC13 cmc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DC13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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