Rabbit anti-Human, Mouse ATG14 Polyclonal Antibody | anti-ATG14 antibody

ATG14, NT (ATG14, KIAA0831, Beclin 1-associated autophagy-related key regulator, Autophagy-related protein 14-like protein) (MaxLight 490)

Cat. Num. AAA6276013

• Gene Names: ATG14; ATG14L; BARKOR; KIAA0831
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: ATG14; Polyclonal Antibody; NT (ATG14; KIAA0831; Beclin 1-associated autophagy-related key regulator; Autophagy-related protein 14-like protein) (MaxLight 490); Autophagy-related protein 14-like protein; anti-ATG14 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-ATG14 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: ATG14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62~92 amino acids from the N-terminal region of human ATG14.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ATG14 antibody (N-term) in mouse heart tissue lysates (35ug/lane). ATG14 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(ATG14 Antibody (N-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ATG14 antibody

MaxLight 490 is a new Blue-Green photostable dye conjugate comparable to DyLight488, Alexa Fluor488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.

Product Categories/Family for anti-ATG14 antibody

Antibodies; Autophagy

References

Sun, Q., Proc. Natl. Acad. Sci. U.S.A. 105 (49), 19211-19216 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 50540545
• NCBI GeneID: 22863
• NCBI Accession #: NP_055739
• NCBI GenBank Nucleotide #: NM_014924.4
• UniProt Accession #: Q6ZNE5; O94920; Q32MK7; Q32MK8; A6NJE4; A8K9U5; B7ZWP5
• Molecular Weight: 54 kDa
• NCBI Official Full Name: beclin 1-associated autophagy-related key regulator
• NCBI Official Synonym Full Names: autophagy related 14
• NCBI Official Symbol: ATG14
• NCBI Official Synonym Symbols: ATG14L; BARKOR; KIAA0831
• NCBI Protein Information: beclin 1-associated autophagy-related key regulator; Beclin 1-Interacting protein; ATG14 autophagy related 14 homolog; autophagy-related protein 14-like protein
• UniProt Protein Name: Beclin 1-associated autophagy-related key regulator
• Protein Family: Autophagy-related protein
• UniProt Gene Name: ATG14
• UniProt Synonym Gene Names: KIAA0831; Barkor; Atg14L
• UniProt Entry Name: BAKOR_HUMAN

Uniprot Description

Barkor: Required for both basal and inducible autophagy. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner. Forms a complex with BECN1, PIK3C3 and PIK3R4, but not with UVRAG, nor with KIAA0226/Rubicon. UVRAG and ATG14/Barkor form mutually exclusive complexes with BECN1 through direct competition. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy. Interacts with PIK3CB. Belongs to the Barkor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy

Chromosomal Location of Human Ortholog: 14q22.3

Cellular Component: nucleoplasm; cytoplasm; pre-autophagosomal structure membrane; autophagic vacuole; phagocytic vesicle; axoneme; nucleus

Molecular Function: protein binding

Biological Process: regulation of protein amino acid phosphorylation; macroautophagy; mitochondrion degradation; endosome to lysosome transport; positive regulation of autophagy; autophagic vacuole formation