Western Blot (WB)
(Sample Data: Western blot analysis of DAPK3 in HeLa (H) and Jurkat (J) whole cell lysates with DAPK antibody at 1 ug/ml.)
Rabbit DAPK3 Polyclonal Antibody | anti-DAPK3 antibody
Anti-DAPK3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA, Immunocytochemistry, Immunofluorescence
Purity
Affinity chromatography
Synonyms
DAPK3; Polyclonal Antibody; Anti-DAPK3; anti-DAPK3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the DAPK3 protein
Purity/Purification
Affinity chromatography
Form/Format
PBS + 0.02% sodium azide
Applicable Applications for anti-DAPK3 antibody
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF)
Immunogen
DAPK3 antibody was raised against a peptide corresponding to amino acids near the center of human DAPK3.
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
1 year at -20 degree C from date of shipment
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western blot analysis of DAPK3 in HeLa (H) and Jurkat (J) whole cell lysates with DAPK antibody at 1 ug/ml.)
Western Blot (WB)
(Sample Data: Western blot analysis of DAPK3 in HeLa (H) and Jurkat (J) whole cell lysates with DAPK antibody at 1 ug/ml.)
Immunocytochemistry (ICC)
(Sample Data: Immunocytochemistry of DAPK3 in Jurkat cells with DAPK3 antibody at 10 ug/ml.)
Immunocytochemistry (ICC)
(Sample Data: Immunocytochemistry of DAPK3 in Jurkat cells with DAPK3 antibody at 10 ug/ml.)
Related Product Information for anti-DAPK3 antibody
DAPK3 or Death-associated protein kinase 3 (also known as ZIP) plays a role in apoptosis (1). DAPK3 is a nuclear serine/threonine-specific kinase that phosphorylates core histones H3 and H4, and myosin light chain in vitro. DAPK3 interacts with transcription and splicing factors as well as with pro-apoptotic protein Par-4 suggesting that it participates in multiple cellular processes. DAPK3 contains a leucine zipper structure at its C terminus and this region is responsible for binding to ATF4. The leucine zipper domain is necessary for the homodimerization of DAPK3 as well as for the activation of the kinase (2).
Product Categories/Family for anti-DAPK3 antibody
References
1. Kawai, T. et al: ZIP kinase, a novel serine/threonine kinase which mediates apoptosis. Mol. Cell Biol. 1998;18(3):1642-51.
2. Preuss, U. et al. Novel mitosis-specific phosphorylation of histone H3 at Thr11 mediated by Dlk/ZIP kinase. Nucleic Acids Res. 2003; 31(3):878-85.
2. Preuss, U. et al. Novel mitosis-specific phosphorylation of histone H3 at Thr11 mediated by Dlk/ZIP kinase. Nucleic Acids Res. 2003; 31(3):878-85.
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Product Notes
The DAPK3 (Catalog #AAA515932) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DAPK3 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DAPK3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the DAPK3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DAPK3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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