Western Blot (WB)
(Sample Data: Western blot analysis of IKK gamma in HeLa whole cell lysate with IKK gamma antibody at 1 ug/ml.)
Rabbit IKK gamma Polyclonal Antibody | anti-IKKgamma antibody
Anti-IKK gamma
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA, Immunocytochemistry
Purity
Affinity chromatography
Synonyms
IKK gamma; Polyclonal Antibody; Anti-IKK gamma; anti-IKKgamma antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the IKK gamma protein and has no cross response to IKK alpha or IKK beta
Purity/Purification
Affinity chromatography
Form/Format
PBS + 0.02% sodium azide
Applicable Applications for anti-IKKgamma antibody
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
Immunogen
IKK gamma antibody was raised against a peptide corresponding to amino acids 400 to 416 of human IKK gamma, which are identical to those of mouse homologue.
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
1 year at -20 degree C from date of shipment
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western blot analysis of IKK gamma in HeLa whole cell lysate with IKK gamma antibody at 1 ug/ml.)
Western Blot (WB)
(Sample Data: Western blot analysis of IKK gamma in HeLa whole cell lysate with IKK gamma antibody at 1 ug/ml.)
Immunocytochemistry (ICC)
(Sample Data: Immunocytochemistry of IKK gamma in HeLa cells with IKK gamma antibody at 5 ug/ml.)
Immunocytochemistry (ICC)
(Sample Data: Immunocytochemistry of IKK gamma in HeLa cells with IKK gamma antibody at 5 ug/ml.)
Related Product Information for anti-IKKgamma antibody
IKKgamma is a serine/threonine protein kinase that phosphorylates the I-kappa-B protein which is an inhibitor of the transcription factor NF-kappa-B complex. Phosphorylation of I-kappa-B protein triggers the degradation of the inhibitor via the ubiquitination pathway, thereby activating NF-kappa-B complex. IKKgamma forms dimers and trimers and interacts preferentially with IKKbeta but not IKKalpha (1). IKKgamma associates with activated ATM after the induction of DNA double-strand breaks (2). ATM phosphorylates serine-85 of IKKgamma to promote its ubiquitin-dependent nuclear export.
Product Categories/Family for anti-IKKgamma antibody
References
1. Rothwarf, D. M. et al: IKK-gamma is an essential regulatory subunit of the I-kappa-B kinase complex. Nature 395: 297-300, 1998.
2. Wu, Z-H. et al: Molecular linkage between the kinase ATM and NF-kappaB signaling in response to genotoxic stimuli. Science 311: 1141-1146, 2006.
2. Wu, Z-H. et al: Molecular linkage between the kinase ATM and NF-kappaB signaling in response to genotoxic stimuli. Science 311: 1141-1146, 2006.
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Product Notes
The IKKgamma (Catalog #AAA516022) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-IKK gamma reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IKK gamma can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the IKKgamma for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IKK gamma, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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