Western Blot (WB)
(Sample Data: Western blot analysis of ROCK1 in 293 cell lysate with ROCK1 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.)
Rabbit ROCK1 Polyclonal Antibody | anti-ROCK1 antibody
Anti-ROCK1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA, Immunocytochemistry, Immunofluorescence
Purity
Affinity chromatography
Synonyms
ROCK1; Polyclonal Antibody; Anti-ROCK1; anti-ROCK1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the ROCK1 protein and will not cross-react with other ROCK family members
Purity/Purification
Affinity chromatography
Form/Format
PBS + 0.02% sodium azide
Applicable Applications for anti-ROCK1 antibody
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF)
Immunogen
ROCK1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human ROCK1
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
1 year at -20 degree C from date of shipment
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western blot analysis of ROCK1 in 293 cell lysate with ROCK1 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.)
Western Blot (WB)
(Sample Data: Western blot analysis of ROCK1 in 293 cell lysate with ROCK1 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.)
Immunocytochemistry (ICC)
(Sample Data: Immunocytochemistry of ROCK1 in 293 cells with ROCK1 antibody at 10 ug/ml.)
Immunocytochemistry (ICC)
(Sample Data: Immunocytochemistry of ROCK1 in 293 cells with ROCK1 antibody at 10 ug/ml.)
Related Product Information for anti-ROCK1 antibody
ROCK1 is a ubiquitously expressed serine/threonine kinase that is a downstream target of the small GTPase RhoA. ROCK1 is involved in diverse cellular functions, including smooth muscle contraction, actin cytoskeleton organization, cell adhesion and motility, and gene expression (1). ROCK1 contributes to the development of cardiac fibrosis and induction of fibrogenic cytokines in cardiomyocytes in response to pathological stimuli. ROCK1 knockout mice exhibit reduced perivascular and interstitial fibrosis which is associated with reduced expression of a variety of extracellular matrix (ECM) proteins and fibrogenic cytokines (2).
Product Categories/Family for anti-ROCK1 antibody
References
1. Zhao, Y M. et al: Rho-associated kinases play a role in endocardial cell differentiation and migration. Dev Biol. 2004 Nov 1;275(1):183-91.
2. Zhang, C. et al: Targeted deletion of ROCK1 protects the heart against pressure overload by inhibiting reactive fibrosis. FASEB J. 2006 May; 20(7):916-25.
2. Zhang, C. et al: Targeted deletion of ROCK1 protects the heart against pressure overload by inhibiting reactive fibrosis. FASEB J. 2006 May; 20(7):916-25.
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Product Notes
The ROCK1 (Catalog #AAA516180) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ROCK1 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ROCK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the ROCK1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ROCK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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