Western Blot (WB)
(Sample Data: Western blot analysis of IKK alpha in HeLa whole cell lysate with IKK alpha antibody at 1:500 dilution.)
Rabbit anti-Human IKK alpha Polyclonal Antibody | anti-IKKalpha antibody
Anti-IKK alpha
Reactivity
Human
Applications
Western Blot, ELISA, Immunocytochemistry, Immunofluorescence
Purity
Affinity chromatography
Synonyms
IKK alpha; Polyclonal Antibody; Anti-IKK alpha; anti-IKKalpha antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the IKK alpha protein and has no cross response to IKK beta or IKK gamma
Purity/Purification
Affinity chromatography
Form/Format
PBS + 0.02% sodium azide
Applicable Applications for anti-IKKalpha antibody
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF)
Immunogen
IKK alpha antibody was raised against a peptide corresponding to amino acids 699 to 715 of human IKK alpha, which differs from corresponding murine sequence by one amino acid.
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
1 year at -20 degree C from date of shipment
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western blot analysis of IKK alpha in HeLa whole cell lysate with IKK alpha antibody at 1:500 dilution.)
Western Blot (WB)
(Sample Data: Western blot analysis of IKK alpha in HeLa whole cell lysate with IKK alpha antibody at 1:500 dilution.)
Immunocytochemistry (ICC)
(Sample Data: Immunocytochemistry of IKK alpha in HeLa cells with IKK alpha antibody at 10 ug/ml.)
Immunocytochemistry (ICC)
(Sample Data: Immunocytochemistry of IKK alpha in HeLa cells with IKK alpha antibody at 10 ug/ml.)
Related Product Information for anti-IKKalpha antibody
IKKalpha is a serine/threonine protein kinase that phosphorylates the I-kappa-B protein which is an inhibitor of the transcription factor NF-kappa-B complex. Phosphorylation of I-kappa-B protein triggers the degradation of the inhibitor via the ubiquitination pathway, thereby activating NF-kappa-B complex (1). IKKalpha is an essential regulator of NF-kappa-B-dependent gene expression through control of promoter-associated histone phosphorylation after cytokine exposure (2). IKKalpha is a critical component of the cytoplasmic transductional-transcriptional processor leading to induction of IFN alpha production. IKKalpha is also involved in the epidermis where it antagonizes mitogenic and angiogenic signals and represses tumor progression and metastases.
Product Categories/Family for anti-IKKalpha antibody
References
1. Anest, V.et.al: A nucleosomal function for I-kappa-B kinase-alpha in NF-kappa-B-dependent gene expression. Nature 423: 659-663, 2003.
2. Hoshino, K. et.al: I-kappa-B kinase-alpha is critical for interferon-alpha production induced by Toll-like receptors 7 and 9. Nature 440: 949-953, 2006.
2. Hoshino, K. et.al: I-kappa-B kinase-alpha is critical for interferon-alpha production induced by Toll-like receptors 7 and 9. Nature 440: 949-953, 2006.
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Product Notes
The IKKalpha (Catalog #AAA515913) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-IKK alpha reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IKK alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the IKKalpha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IKK alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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