Rabbit anti-Human DAPK2 Polyclonal Antibody | anti-DAPK2 antibody

DAPK2 Antibody

Cat. Num. AAA7006583

• Gene Names: DAPK2; DRP1; DRP-1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Caprylic Acid Ammonium Sulfate Precipitation purified
• Synonyms: DAPK2; Polyclonal Antibody; DAPK2 Antibody; DAP kinase 2; DAP-kinase-related protein 1; DRP-1; anti-DAPK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Caprylic Acid Ammonium Sulfate Precipitation purified
• Form/Format: Liquid
• Sequence Length: 370

• Applicable Applications for anti-DAPK2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: WB:1:500-2000, IHC:1:20-1:200
• Immunogen: Recombinant human Death-associated protein kinase 2 protein(222-370AA)
• Conjugation: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-10084 / sc-10081 / sc-130734
• Preparation and Storage: Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western blotAll lanes: DAPK2 antibody at 6ug/ml+ Mouse brain tissueGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 43,56 kDaObserved band size: 43 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using MBS7006583 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human breast cancer tissue using MBS7006583 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver cancer tissue using MBS7006583 at dilution 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of MCF-7 cells using MBS7006583 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-DAPK2 antibody

Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation. Isoform 2 is not regulated by calmodulin. It can phosphorylate MYL9. It can induce membrane blebbing and autophagic cell death.

NCBI and Uniprot Product Information

• NCBI GI #: 14670383
• NCBI GeneID: 23604
• NCBI Accession #: NP_055141.2
• NCBI GenBank Nucleotide #: NM_014326.3
• UniProt Accession #: Q9UIK4; O75892; Q24JS1; E9JGM7
• Molecular Weight: 55,931 Da
• NCBI Official Full Name: death-associated protein kinase 2
• NCBI Official Synonym Full Names: death-associated protein kinase 2
• NCBI Official Symbol: DAPK2
• NCBI Official Synonym Symbols: DRP1; DRP-1
• NCBI Protein Information: death-associated protein kinase 2
• UniProt Protein Name: Death-associated protein kinase 2
• Protein Family: Death-associated protein kinase
• UniProt Gene Name: DAPK2
• UniProt Synonym Gene Names: DAP kinase 2; DRP-1
• UniProt Entry Name: DAPK2_HUMAN

NCBI Description

This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq, Jul 2008]

Uniprot Description

DAPK2: an ubiquitously expressed protein kinase of the DAPK family. Activated by Ca(2+)/calmodulin. Contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain. Overexpression induces apoptosis. Expressed at high levels in heart, lung and skeletal muscle.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC 2.7.11.1; CAMK group; DAPK family

Chromosomal Location of Human Ortholog: 15q22.31

Cellular Component: cytoplasm; cytoplasmic vesicle

Molecular Function: ATP binding; calmodulin binding; identical protein binding; protein binding; protein serine/threonine kinase activity

Biological Process: anoikis; apoptosis; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of apoptosis; regulation of autophagy