Mouse anti-Human DAPK2 / DAP Kinase 2 Monoclonal Antibody | anti-DAPK2 antibody

Anti-DAPK2 / DAP Kinase 2 Antibody (clone 1B5) IHC-plus

Cat. Num. AAA249398

• Gene Names: DAPK2; DRP1; DRP-1
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, Immunoprecipitation
• Purity: Protein A/G purified
• Synonyms: DAPK2 / DAP Kinase 2; Monoclonal Antibody; Anti-DAPK2 / DAP Kinase 2 Antibody (clone 1B5) IHC-plus; Mouse Monoclonal [clone 1B5] (IgG2b) to Human DAPK2 / DAP Kinase 2; Human DAPK2 / DAP Kinase 2; DAP-kinase-related protein 1; DRP-1; DRP1; DAP kinase 2; anti-DAPK2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 1B5
• Specificity: Human DAPK2
• Purity/Purification: Protein A/G purified
• Form/Format: PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 370

• Applicable Applications for anti-DAPK2 antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: IF (1:100); IHC-P (10 ug/ml); WB (1:2000)
• Immunogen: DAPK2 / DAP Kinase 2 antibody was raised against full length human recombinant protein of human DAPK2 (NP_055141) produced in HEK293T cell.
• Immunogen Description: Full length human recombinant protein of human DAPK2 (NP_055141) produced in HEK293T cell.
• Immunogen Type: Recombinant protein
• Target Species: Human
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-DAPK2 / DRP1 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Anti-DAPK2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DAPK2.)

Western Blot (WB)

(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAPK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAPK2.)

Immunoprecipitation (IP)

(Immunoprecipitation(IP) of DAPK2 by using monoclonal anti-DAPK2 antibodies (Negative control: IP without adding anti-DAPK2 antibody.). For each experiment, 500ul of DDK tagged DAPK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-DAPK2 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.)

Related Product Information for anti-DAPK2 antibody

DAPK2 Antibody, DAP-kinase-related protein 1 Antibody, DRP-1 Antibody, DRP1 Antibody, DAP kinase 2 Antibody Description: Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles.

NCBI and Uniprot Product Information

• NCBI GI #: 14670383
• NCBI GeneID: 23604
• NCBI Accession #: NP_055141.2
• NCBI GenBank Nucleotide #: NM_014326.3
• UniProt Accession #: Q9UIK4; O75892; Q24JS1; E9JGM7
• Molecular Weight: 55,931 Da
• NCBI Official Full Name: death-associated protein kinase 2
• NCBI Official Synonym Full Names: death-associated protein kinase 2
• NCBI Official Symbol: DAPK2
• NCBI Official Synonym Symbols: DRP1; DRP-1
• NCBI Protein Information: death-associated protein kinase 2
• UniProt Protein Name: Death-associated protein kinase 2
• Protein Family: Death-associated protein kinase
• UniProt Gene Name: DAPK2
• UniProt Synonym Gene Names: DAP kinase 2; DRP-1
• UniProt Entry Name: DAPK2_HUMAN

NCBI Description

This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq, Jul 2008]

Uniprot Description

DAPK2: an ubiquitously expressed protein kinase of the DAPK family. Activated by Ca(2+)/calmodulin. Contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain. Overexpression induces apoptosis. Expressed at high levels in heart, lung and skeletal muscle.

Protein type: EC 2.7.11.1; Protein kinase, CAMK; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; DAPK family

Chromosomal Location of Human Ortholog: 15q22.31

Cellular Component: cytoplasm; cytoplasmic vesicle

Molecular Function: ATP binding; calmodulin binding; identical protein binding; protein binding; protein serine/threonine kinase activity

Biological Process: anoikis; apoptosis; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of apoptosis; regulation of autophagy