Mouse anti-Human XRN2 Monoclonal Antibody | anti-XRN2 antibody

XRN2 Antibody

Cat. Num. AAA9435268

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: ProA affinity purified
• Synonyms: XRN2; Monoclonal Antibody; XRN2 Antibody; 5' 3' exoribonuclease 2 antibody; DHM1 like protein antibody; DHP protein antibody; XRN 2 antibody; anti-XRN2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Purity/Purification: ProA affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-XRN2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:200-1:500
• Immunogen: Recombinant protein
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of XRN2 on human XRN2 recombinant protein using anti-XRN2 antibody at 1/1, 000 dilution.)

Western Blot (WB)

(Western blot analysis of XRN2 on HEK293 (1) and XRN2-hIgGFc transfected HEK293 (2) cell lysate using anti-XRN2 antibody at 1/1, 000 dilution.)

Western Blot (WB)

(Western blot analysis of XRN2 on different cell lysate using anti-XRN2 antibody at 1/1, 000 dilution. Positive control�� Lane1: Raw264.7 Lane2: HEK293 Lane3: NTERA-2 Lane4: LNcap Lane5: HepG2 Lane6: HEK293 Lane7: Hela )

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-XRN2 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti- XRN2 antibody. Counter stained with hematoxylin.)

Related Product Information for anti-XRN2 antibody

Degradation of mRNA is a critical aspect of gene expression that occurs via the exoribonuclease. Exoribonuclease 2 (XRN2) is the human homologue of the Saccharomyces cerevisiae RAT1, which functions as a nuclear 5' to 3' exoribonuclease and is essential for mRNA turnover and cell viability. XRN2 also processes rRNAs and small nucleolar RNAs (snoRNAs) in the nucleus. XRN2 moves along with RNA polymerase II and gains access to the nascent RNA transcript after the endonucleolytic cleavage at the poly (A) site or at a second cotranscriptional cleavage site (CoTC). CoTC is an autocatalytic RNA structure that undergoes rapid self-cleavage and acts as a precursor to termination by presenting a free RNA 5' end to be recognized by XRN2. XRN2 then travels in a 5'-3' direction like a guided torpedo and facilitates the dissociation of the RNA polymerase elongation complex.

Product Categories/Family for anti-XRN2 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 18860916
• NCBI GeneID: 22803
• NCBI Accession #: NP_036387.2
• NCBI GenBank Nucleotide #: NM_012255.3
• UniProt Accession #: Q9H0D6; Q3L8N4; Q6KGZ9; Q9BQL1; Q9NTW0; Q9NXS6; Q9UL53
• Molecular Weight: 108,582 Da
• NCBI Official Full Name: 5'-3' exoribonuclease 2
• NCBI Official Synonym Full Names: 5'-3' exoribonuclease 2
• NCBI Official Symbol: XRN2
• NCBI Protein Information: 5'-3' exoribonuclease 2; DHP protein; Dhm1-like protein (mouse homolog)
• UniProt Protein Name: 5'-3' exoribonuclease 2
• UniProt Gene Name: XRN2
• UniProt Synonym Gene Names: DHP protein
• UniProt Entry Name: XRN2_HUMAN

NCBI Description

This gene shares similarity with the mouse Dhm1 and the yeast dhp1 gene. The yeast gene is involved in homologous recombination and RNA metabolism, such as RNA synthesis and RNA trafficking. Complementation studies show that Dhm1 has a similar function in mouse as dhp1. The function of the human gene has not yet been determined. Transcript variants encoding different isoforms have been noted for this gene; however, their full-length nature is not known. [provided by RefSeq, Jul 2008]

Uniprot Description

XRN2: Possesses 5'->3' exoribonuclease activity. May promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription. Belongs to the 5'-3' exonuclease family. XRN2/RAT1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.13.-; Ribonuclease; RNA processing; RNA-binding; Cell development/differentiation; Nucleolus

Chromosomal Location of Human Ortholog: 20p11.2-p11.1

Cellular Component: membrane; nucleolus; nucleus

Molecular Function: 5'-3' exonuclease activity; 5'-3' exoribonuclease activity; nuclease activity; zinc ion binding

Biological Process: RNA processing; regulation of transcription, DNA-dependent; RNA metabolic process; RNA catabolic process; spermatogenesis; cell growth; mRNA processing; DNA catabolic process, exonucleolytic; transcription termination