Rabbit anti-Human Dapk1 Polyclonal Antibody | anti-Dapk1 antibody

Dapk1, NT (Dapk1, Death-associated protein kinase 1) (APC)

Cat. Num. AAA6290783

• Gene Names: Dapk1; D13Ucla1; DAP-Kinase
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Dapk1; Polyclonal Antibody; NT (Dapk1; Death-associated protein kinase 1) (APC); Death-associated protein kinase 1; anti-Dapk1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-Dapk1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Dapk1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 384-412 amino acids from the N-terminal region of Mouse Dapk1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Dapk1 Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the Dapk1 antibody detected the Dapk1 protein (arrow).)

Related Product Information for anti-Dapk1 antibody

Calcium/calmodulin-dependent serine/threonine kinase. Isoform 1 is a negative regulator of apoptosis in direct contrast to its human homolog. Isoform 2 has no effect on apoptosis and its function is unknown.

Product Categories/Family for anti-Dapk1 antibody

Antibodies; Protein Kinases

References

Tu, W., et al. Cell 140(2):222-234(2010). Kang, B.N., et al. J. Neurosci. 30(1):93-98(2010). Munger, S.C., et al. Genes Dev. 23(21):2521-2536(2009). Li, L., et al. Int. J. Oncol. 34(4):905-914(2009). Stevens, C., et al. J. Biol. Chem. 284(1):334-344(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 114205418
• NCBI GeneID: 69635
• NCBI Accession #: NP_083929.2
• NCBI GenBank Nucleotide #: NM_029653.3
• UniProt Accession #: Q80YE7; Q80YE6; Q8R341; Q8VDN6; Q9CSD4; Q9JJP7
• Molecular Weight: 161442
• NCBI Official Full Name: death-associated protein kinase 1 isoform 1
• NCBI Official Synonym Full Names: death associated protein kinase 1
• NCBI Official Symbol: Dapk1
• NCBI Official Synonym Symbols: D13Ucla1; DAP-Kinase
• NCBI Protein Information: death-associated protein kinase 1
• UniProt Protein Name: Death-associated protein kinase 1
• Protein Family: Death-associated protein kinase
• UniProt Gene Name: Dapk1
• UniProt Synonym Gene Names: DAP kinase 1

Uniprot Description

Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3 (). Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca2+ influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition.