Rabbit anti-Human Dapk1 Polyclonal Antibody | anti-DAPK1 antibody

Dapk1, NT (Dapk1, Death-associated protein kinase 1)

Cat. Num. AAA647380

• Gene Names: DAPK1; DAPK
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Dapk1; Polyclonal Antibody; NT (Dapk1; Death-associated protein kinase 1); Anti -Dapk1; anti-DAPK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-DAPK1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: Mouse Dapk1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 384-412 amino acids from the N-terminal region of Mouse Dapk1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Dapk1 Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the Dapk1 antibody detected the Dapk1 protein (arrow).)

Related Product Information for anti-DAPK1 antibody

Calcium/calmodulin-dependent serine/threonine kinase. Isoform 1 is a negative regulator of apoptosis in direct contrast to its human homolog. Isoform 2 has no effect on apoptosis and its function is unknown.

Product Categories/Family for anti-DAPK1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 89363047
• NCBI GeneID: 1612
• NCBI Accession #: NP_004929.2
• NCBI GenBank Nucleotide #: NM_004938.2
• UniProt Accession #: P53355; Q14CQ7; Q1W5W0; Q68CP8; Q6ZRZ3; Q9BTL8; B7ZLD2
• Molecular Weight: 160,046 Da
• NCBI Official Full Name: death-associated protein kinase 1
• NCBI Official Synonym Full Names: death-associated protein kinase 1
• NCBI Official Symbol: DAPK1
• NCBI Official Synonym Symbols: DAPK
• NCBI Protein Information: death-associated protein kinase 1; DAP kinase 1
• UniProt Protein Name: Death-associated protein kinase 1
• Protein Family: Death-associated protein kinase
• UniProt Gene Name: DAPK1
• UniProt Synonym Gene Names: DAPK; DAP kinase 1
• UniProt Entry Name: DAPK1_HUMAN

NCBI Description

Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca2+ influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition. Ref.1 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.21 Ref.22 Ref.23 Ref.24 Ref.25 Ref.26 Ref.27 Ref.30 Ref.31Isoform 2 cannot induce apoptosis but can induce membrane blebbing. Ref.1 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.21 Ref.22 Ref.23 Ref.24 Ref.25 Ref.26 Ref.27 Ref.30 Ref.31

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium.

Enzyme regulation: Activated by Ca2+/calmodulin. Regulated by a locking mechanism, involving autophosphorylation at Ser-308 and calmodulin binding. In the inactive state, Ser-308 is phosphorylated. Activation involves its dephosphorylation and a release-of-autoinhibition mechanism where binding of calmodulin induces a conformational change that relieves the steric block of the active site by the autoinhibitory domain. Activity is modulated by UNC5B and NTN1. UNC5B activates it by inhibiting the phosphorylation at Ser-308, whereas NTN1 inhibits UNC5B-mediated activation of DAPK1. Endoplasmic-stress activates by causing Ser-308 dephosphorylation. Ref.11 Ref.18 Ref.20

Subunit structure: Interacts with KLHL20. Interacts (via death domain) with MAPK1 and MAPK3. Interacts with MAP1B (via N-terminus). Interacts (via death domain) with UNC5B (via death domain). Interacts with PRKD1 in an oxidative stress-regulated manner. Interacts with PIN1, PDCD6, BECN1, GRINB, TSC2 and STX1A. Interacts (via kinase domain) with DAPK3 (via kinase domain). Ref.13 Ref.14 Ref.15 Ref.17 Ref.18 Ref.21 Ref.24 Ref.26 Ref.27 Ref.28 Ref.30

Subcellular location: Isoform 1: Cytoplasm. Cytoplasm › cytoskeleton Ref.10 Ref.12 Ref.23 Ref.24. Note: Colocalizes with MAP1B in the microtubules and cortical actin fibers. Ref.10 Ref.12 Ref.23 Ref.24Isoform 2: Cytoplasm. Cytoplasm › cytoskeleton Ref.10 Ref.12 Ref.23 Ref.24.

Tissue specificity: Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas. Ref.23

Induction: Up-regulated following treatment with IFNG/IFN-gamma. Ref.1 Ref.11 Ref.18 Ref.20

Domain: The autoinhibitory domain sterically blocks the substrate peptide-binding site by making both hydrophobic and electrostatic contacts with the kinase core.

Post-translational modification: Ubiquitinated by the BCR(KLHL20) E3 ubiquitin ligase complex, leading to its degradation by the proteasome. Ref.28Removal of the C-terminal tail of isoform 2 (corresponding to amino acids 296-337 of isoform 2) by proteolytic cleavage stimulates maximally its membrane-blebbing function.In response to mitogenic stimulation (PMA or EGF), phosphorylated at Ser-289; phosphorylation suppresses DAPK1 pro-apoptotic function. Autophosphorylation at Ser-308 inhibits its catalytic activity. Phosphorylation at Ser-734 by MAPK1 increases its catalytic activity and promotes cytoplasmic retention of MAPK1. Endoplasmic-stress can cause dephosphorylation at Ser-308. Ref.11 Ref.16 Ref.17 Ref.18 Ref.20

Sequence similarities: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.Contains 10 ANK repeats.Contains 1 death domain.Contains 1 protein kinase domain.

Sequence caution: The sequence AAP35581.1 differs from that shown. Reason: Contaminating sequence. Sequence of unknown origin in the C-terminal part.The sequence CAA53712.1 differs from that shown. Reason: Frameshift at positions 462 and 464.