Mouse Death Associated Protein Kinase 1 (DAPK1) ELISA Kit | DAPK1 elisa kit

Mouse Death Associated Protein Kinase 1 (DAPK1) ELISA Kit

Cat. Num. AAA453807

• Gene Names: Dapk1; D13Ucla1; DAP-Kinase
• Reactivity: Mouse
• Synonyms: Death Associated Protein Kinase 1 (DAPK1); Mouse Death Associated Protein Kinase 1 (DAPK1) ELISA Kit; DAPK1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of DAPK1. No significant cross-reactivity or interference between DAPK1 and analogues was observed.
• Sequence Length: 1442

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.051ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DAPK1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DAPK1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DAPK1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DAPK1 in mouse tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DAPK1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DAPK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DAPK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DAPK1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 552953823
• NCBI GeneID: 69635
• NCBI Accession #: NP_001272846.1
• NCBI GenBank Nucleotide #: NM_001285917.1
• UniProt Accession #: Q80YE7; Q80YE6; Q8R341; Q8VDN6; Q9CSD4; Q9JJP7
• Molecular Weight: 159,986 Da
• NCBI Official Full Name: death-associated protein kinase 1 isoform 2
• NCBI Official Synonym Full Names: death associated protein kinase 1
• NCBI Official Symbol: Dapk1
• NCBI Official Synonym Symbols: D13Ucla1; DAP-Kinase
• NCBI Protein Information: death-associated protein kinase 1
• UniProt Protein Name: Death-associated protein kinase 1
• Protein Family: Death-associated protein kinase
• UniProt Gene Name: Dapk1
• UniProt Synonym Gene Names: DAP kinase 1

Uniprot Description

Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3 (). Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca2+ influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition.