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Western Blot (WB) (Western Blot analysis of 293 cells using Elf-1 Polyclonal Antibody)

Rabbit anti-Human Elf-1 Polyclonal Antibody | anti-Elf-1 antibody

Elf-1 Polyclonal Antibody

Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Elf-1; Polyclonal Antibody; Elf-1 Polyclonal Antibody; ELF1; ETS-related transcription factor Elf-1; E74-like factor 1; anti-Elf-1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Elf-1 Polyclonal Antibody detects endogenous levels of Elf-1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
595
Applicable Applications for anti-Elf-1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:100 - 1:300
Immunofluorescence: 1:200 - 1:1000
ELISA: 1:20000
Not yet tested in other applications.
Immunogen Type
peptide
Immunogen Description
Synthesized peptide derived from the C-terminal region of human Elf-1.
Target Name
Elf-1
Preparation and Storage
Store at -20 degree C/1 year

Western Blot (WB)

(Western Blot analysis of 293 cells using Elf-1 Polyclonal Antibody)

Western Blot (WB) (Western Blot analysis of 293 cells using Elf-1 Polyclonal Antibody)
Product Categories/Family for anti-Elf-1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
64,929 Da
NCBI Official Full Name
ETS-related transcription factor Elf-1 isoform b
NCBI Official Synonym Full Names
E74-like factor 1 (ets domain transcription factor)
NCBI Official Symbol
ELF1
NCBI Protein Information
ETS-related transcription factor Elf-1
UniProt Protein Name
ETS-related transcription factor Elf-1
UniProt Gene Name
ELF1
UniProt Entry Name
ELF1_HUMAN

NCBI Description

This gene encodes an E26 transformation-specific related transcription factor. The encoded protein is primarily expressed in lymphoid cells and acts as both an enhancer and a repressor to regulate transcription of various genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2009]

Uniprot Description

Elf-1: Transcription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer. Belongs to the ETS family.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 13q13

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; negative regulation of T cell receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; cell differentiation; regulation of cytokine production

Research Articles on Elf-1

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Product Notes

The Elf-1 elf1 (Catalog #AAA9401077) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Elf-1 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Elf-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:100 - 1:300 Immunofluorescence: 1:200 - 1:1000 ELISA: 1:20000 Not yet tested in other applications. Researchers should empirically determine the suitability of the Elf-1 elf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Elf-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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