Western Blot (WB)
(DACT3 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the DACT3 antibody detected the DACT3 protein (arrow).)
Rabbit DACT3 Polyclonal Antibody | anti-DACT3 antibody
DACT3 Antibody (N-term)
Gene Names
DACT3; RRR1; DAPPER3
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DACT3; Polyclonal Antibody; DACT3 Antibody (N-term); Dapper homolog 3; Antagonist of beta-catenin Dapper homolog 3; Arginine-rich region 1 protein; Dapper antagonist of catenin 3; RRR1; anti-DACT3 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DACT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 139-165 amino acids from the N-terminal region of human DACT3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
139-165
Sequence Length
629
Applicable Applications for anti-DACT3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(DACT3 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the DACT3 antibody detected the DACT3 protein (arrow).)
Western Blot (WB)
(DACT3 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the DACT3 antibody detected the DACT3 protein (arrow).)
Related Product Information for anti-DACT3 antibody
Dact3 is the gene product of the third discovered member of the Dact (Dapper) family of developmentally-expressed genes. Discovered in mouse, a highly conserved ortholog also exists in the human genome. As with other Dact family members, Dact3 may play a role in antagonism of Wnt signaling.
Product Categories/Family for anti-DACT3 antibody
References
Jiang, X., et al. Cancer Cell 13(6):529-541(2008)
Fisher, D.A., et al. Dev. Dyn. 235(9):2620-2630(2006)
Fisher, D.A., et al. Dev. Dyn. 235(9):2620-2630(2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
64949
NCBI Official Full Name
dapper homolog 3 isoform 1
NCBI Official Synonym Full Names
dishevelled-binding antagonist of beta-catenin 3
NCBI Official Symbol
DACT3
NCBI Official Synonym Symbols
RRR1; DAPPER3
NCBI Protein Information
dapper homolog 3
UniProt Protein Name
Dapper homolog 3
Protein Family
UniProt Gene Name
DACT3
UniProt Synonym Gene Names
RRR1
UniProt Entry Name
DACT3_HUMAN
Uniprot Description
DACT3: May be involved in regulation of intracellular signaling pathways during development. Specifically thought to play a role in canonical and/or non-canonical Wnt signaling pathways through interaction with DSH (Dishevelled) family proteins. Belongs to the dapper family.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 19q13.32
Cellular Component: cytoplasm
Molecular Function: identical protein binding; protein kinase C binding; protein kinase A binding
Biological Process: negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; negative regulation of cell growth
Research Articles on DACT3
Similar Products
Product Notes
The DACT3 dact3 (Catalog #AAA9200847) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 139-165. The DACT3 Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's DACT3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the DACT3 dact3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DACT3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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