Rabbit anti-Human Homer 1 Polyclonal Antibody | anti-HOMER1 antibody

Homer 1, NT (Homer Protein Homolog 1, HOMER1, Homer-1, HOMER, Homer1A, Homer1B, Homer1C, SYN47, Ves-1) (MaxLight 405)

Cat. Num. AAA6481090

• Gene Names: HOMER1; HOMER; SYN47; Ves-1; HOMER1A; HOMER1B; HOMER1C
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Homer 1; Polyclonal Antibody; NT (Homer Protein Homolog 1; HOMER1; Homer-1; HOMER; Homer1A; Homer1B; Homer1C; SYN47; Ves-1) (MaxLight 405); Homer Homolog 1 (Drosophila); anti-HOMER1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HOMER1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-HOMER1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human HOMER1 (KLH).
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HOMER1 antibody (N-term) in HepG2 cell line lysates (35ug/lane). HOMER1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma with HOMER1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(HOMER1 Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-HOMER1 antibody

Antibodies; Neuroscience

References

1. Sanna, S., Jackson, A.U. Nat. Genet. 40(2), 198-203 (2008). 2. Dahl, J.P., Kampman, K.M. Psychiatr. Genet. 15(4), 277-283 (2005). 3. Tu, J.C., Xiao, B. Neuron 23(3), 583-592 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 4759198
• NCBI GeneID: 9456
• NCBI Accession #: NP_004263
• NCBI GenBank Nucleotide #: NM_004272.4
• UniProt Accession #: Q86YM7; O96003; Q86YM5; B2R688
• Molecular Weight: 19,861 Da
• NCBI Official Full Name: homer protein homolog 1 isoform 1
• NCBI Official Synonym Full Names: homer scaffolding protein 1
• NCBI Official Symbol: HOMER1
• NCBI Official Synonym Symbols: HOMER; SYN47; Ves-1; HOMER1A; HOMER1B; HOMER1C
• NCBI Protein Information: homer protein homolog 1
• UniProt Protein Name: Homer protein homolog 1
• Protein Family: Homer protein
• UniProt Gene Name: HOMER1
• UniProt Synonym Gene Names: SYN47; Homer-1
• UniProt Entry Name: HOME1_HUMAN

NCBI Description

This gene encodes a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function. [provided by RefSeq, Jul 2008]

Uniprot Description

HOMER1: Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. Interacts with IFT57. Interacts with OPHN1. Isoform 1 encodes a coiled-coil structure that mediates homo- and heteromultimerization. Belongs to the Homer family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5q14.2

Cellular Component: postsynaptic membrane; costamere; cell soma; axon; apical part of cell; excitatory synapse; dendritic shaft; Z disc; cell junction

Molecular Function: identical protein binding; metabotropic glutatmate receptor binding; protein heterodimerization activity; type 5 metabotropic glutamate receptor binding; protein complex scaffold

Biological Process: skeletal muscle contraction; circadian rhythm; response to nicotine; synaptic transmission; positive regulation of signal transduction; chemical homeostasis within a tissue; behavioral response to cocaine; metabotropic glutamate receptor, phospholipase C activating pathway; skeletal muscle fiber development; response to calcium ion; positive regulation of calcium ion transport