Rabbit anti-Human D4-GDI Polyclonal Antibody | anti-D4-GDI antibody

D4-GDI Antibody

Cat. Num. AAA841407

• Gene Names: ARHGDIB; D4; GDIA2; GDID4; LYGDI; Ly-GDI; RAP1GN1; RhoGDI2
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: D4-GDI; Polyclonal Antibody; D4-GDI Antibody; ARHGDIB; 602843; P52566; D4; GDIA2; GDID4; LYGDI; Ly-GDI; RAP1GN1; anti-D4-GDI antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 201

• Applicable Applications for anti-D4-GDI antibody: Western Blot (WB)
• Application Notes: Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 28 kDa D4-GDI in human samples. Jurkat cell lysate can be used as a positive control.
• Immunogen: Synthetic peptide near the N-terminus of human D4-GDI
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-D4-GDI antibody

Background: D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeleytal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block thromugh its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28kD mature form of D4-GDI to give a 23kDa and 5kDa size fragment. The 23kDa fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.

NCBI and Uniprot Product Information

• NCBI GI #: 56676393
• NCBI GeneID: 397
• NCBI Accession #: NP_001166.3
• NCBI GenBank Nucleotide #: NM_001175.5
• UniProt Accession #: P52566; B5BU79
• Molecular Weight: 22,988 Da
• NCBI Official Full Name: rho GDP-dissociation inhibitor 2
• NCBI Official Synonym Full Names: Rho GDP dissociation inhibitor (GDI) beta
• NCBI Official Symbol: ARHGDIB
• NCBI Official Synonym Symbols: D4; GDIA2; GDID4; LYGDI; Ly-GDI; RAP1GN1; RhoGDI2
• NCBI Protein Information: rho GDP-dissociation inhibitor 2
• UniProt Protein Name: Rho GDP-dissociation inhibitor 2
• UniProt Gene Name: ARHGDIB
• UniProt Synonym Gene Names: GDIA2; GDID4; RAP1GN1; Rho GDI 2
• UniProt Entry Name: GDIR2_HUMAN

NCBI Description

Members of the Rho (or ARH) protein family (see MIM 165390) and other Ras-related small GTP-binding proteins (see MIM 179520) are involved in diverse cellular events, including cell signaling, proliferation, cytoskeletal organization, and secretion. The GTP-binding proteins are active only in the GTP-bound state. At least 3 classes of proteins tightly regulate cycling between the GTP-bound and GDP-bound states: GTPase-activating proteins (GAPs), guanine nucleotide-releasing factors (GRFs), and GDP-dissociation inhibitors (GDIs). The GDIs, including ARHGDIB, decrease the rate of GDP dissociation from Ras-like GTPases (summary by Scherle et al., 1993 [PubMed 8356058]).[supplied by OMIM, Dec 2010]

Uniprot Description

RhoGDI beta: regulates the GDP/GTP exchange reaction of Rho proteins by inhibiting the dissociation of GDP from them, keeping these small GTPases in an inactive state. The GDIs are made up of two domains: a flexible N-terminal domain of about 70 amino acid residues and a folded 134-residue C-terminal domain. Regulates the GTP/GDP cycling of glucose-responsive small GTPases. Its phosphorylation on Y156, S101, and S174 mediates the cycling of Cdc42 and Rac1 to regulate second-phase insulin secretion. Interacts with WNK1.

Protein type: G protein regulator, misc.; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 12p12.3

Cellular Component: cytoskeleton; cytoplasm; cytoplasmic membrane-bound vesicle; cytosol

Molecular Function: Rho GDP-dissociation inhibitor activity; GTPase activator activity

Biological Process: regulation of small GTPase mediated signal transduction; regulation of catalytic activity; multicellular organismal development; small GTPase mediated signal transduction; immune response; negative regulation of cell adhesion; cell motility; actin cytoskeleton organization and biogenesis; Rho protein signal transduction; positive regulation of GTPase activity