Rabbit anti-Human, Mouse Cytochrome P450 1A2 Polyclonal Antibody | anti-CYP1A2 antibody

Cytochrome P450 1A2 Antibody

Cat. Num. AAA9408948

• Gene Names: CYP1A2; CP12; P3-450; P450(PA)
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: Cytochrome P450 1A2; Polyclonal Antibody; Cytochrome P450 1A2 Antibody; CP12; CP1A2; EC 1.14.14.1; P3-450; P450(PA); anti-CYP1A2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total Cytochrome P450 1A2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 516

• Applicable Applications for anti-CYP1A2 antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from internal of human Cytochrome P450 1A2.
• Target Name: Cytochrome P450 1A2
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human brain tissue using Cytochrome P450 1A2 antibody.)

Related Product Information for anti-CYP1A2 antibody

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin.Jaiswal A.K., Nucleic Acids Res. 14:6773-6774(1986).Corchero J., Pharmacogenetics 11:1-6(2001).Soyama A.,Drug Metab. Pharmacokinet. 20:24-33(2005).

Product Categories/Family for anti-CYP1A2 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 73915100
• NCBI GeneID: 1544
• NCBI Accession #: NP_000752.2
• NCBI GenBank Nucleotide #: NM_000761.4
• UniProt Accession #: P05177; Q16754; Q6NWU5; Q9BXX7; Q9UK49
• Molecular Weight: 58,407 Da
• NCBI Official Full Name: cytochrome P450 1A2
• NCBI Official Synonym Full Names: cytochrome P450, family 1, subfamily A, polypeptide 2
• NCBI Official Symbol: CYP1A2
• NCBI Official Synonym Symbols: CP12; P3-450; P450(PA)
• NCBI Protein Information: cytochrome P450 1A2
• UniProt Protein Name: Cytochrome P450 1A2
• Protein Family: Cytochrome
• UniProt Gene Name: CYP1A2
• UniProt Entry Name: CP1A2_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP1A2: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O- deethylation of phenacetin. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - linoleic acid; Amino Acid Metabolism - tryptophan; EC 1.14.14.1; Secondary Metabolites Metabolism - caffeine; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450; Cofactor and Vitamin Metabolism - retinol

Chromosomal Location of Human Ortholog: 15q24.1

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle

Molecular Function: enzyme binding; electron carrier activity; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; heme binding; oxidoreductase activity; monooxygenase activity; demethylase activity

Biological Process: methylation; dibenzo-p-dioxin metabolic process; cellular respiration; monoterpenoid metabolic process; epoxygenase P450 pathway; toxin biosynthetic process; response to lipopolysaccharide; monocarboxylic acid metabolic process; response to estradiol stimulus; post-embryonic development; drug catabolic process; regulation of gene expression; xenobiotic metabolic process; porphyrin metabolic process; exogenous drug catabolic process; arachidonic acid metabolic process; alkaloid metabolic process; heterocycle metabolic process; drug metabolic process; hydrogen peroxide biosynthetic process; steroid catabolic process; lung development