Western Blot (WB)
(Western blot analysis of Cytochrome P450 1A2 Antibody expression in rat brain tissue lysates.The lane on the right is treated with the antigen-specific peptide.)
Rabbit Cytochrome P450 1A2 Polyclonal Antibody | anti-CYP1A2 antibody
Cytochrome P450 1A2 Antibody
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
ELISA(peptide): 1:20000-1:40000
IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Western Blot (WB)
(Western blot analysis of Cytochrome P450 1A2 Antibody expression in rat brain tissue lysates.The lane on the right is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of Cytochrome P450 1A2 Antibody expression in rat brain tissue lysates.The lane on the right is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC)
(MBS9605022 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody at 4°C overnight. An HRP conjugated conjugated anti-rabbit Ab was used as secondary Ab)
Immunohistochemistry (IHC)
(MBS9605022 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody at 4°C overnight. An HRP conjugated conjugated anti-rabbit Ab was used as secondary Ab)
Western Blot (WB)
(Western blot analysis of extracts from Myeloma cells, using Cytochrome P450 1A2 Antibody. Lane 1 was treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Myeloma cells, using Cytochrome P450 1A2 Antibody. Lane 1 was treated with the antigen-specific peptide.)
Similarity: Belongs to the cytochrome P450 family.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region. [provided by RefSeq, Jul 2008]
Uniprot Description
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin.
Research Articles on CYP1A2
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Product Notes
The CYP1A2 cyp1a2 (Catalog #AAA9605022) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cytochrome P450 1A2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. WB 1:500-1:1000 IHC: 1:50-1:200 IF/ICC: 1:100-1:500 ELISA(peptide): 1:20000-1:40000 IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Researchers should empirically determine the suitability of the CYP1A2 cyp1a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome P450 1A2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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