Rabbit Cytochrome c Polyclonal Antibody | anti-Cyct antibody

Cytochrome c Antibody

Cat. Num. AAA841048

• Gene Names: Cyct; CYCTA
• Reactivity: Human, Mouse, Rat, Bovine, Chicken, Dog
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity purified
• Synonyms: Cytochrome c; Polyclonal Antibody; Cytochrome c Antibody; CYCS; CYC; anti-Cyct antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Bovine, Chicken, Dog
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.2 mg/ml) affinity purified rabbit anti-Cytochrome c polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.2 mg/ml (varies by lot)
• Sequence Length: 105

• Applicable Applications for anti-Cyct antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Western blotting (0.5-4 ug/ml) and immunoprecipitation (10-20 ug/ml). The cytochrome c antibody detects the 12.6 kDa cytochrome c from human, mouse, and rat samples. Jurkat cell lysate, NIH3T3 cell lysate, and rat kidney tissue lysate can be used as positive controls.
• Immunogen: Synthetic peptide corresponding to residues surrounding amino acid 70 of rat cytochrome c
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-Cyct antibody

Background: Cytochrome c (m.w. 12,500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases

NCBI and Uniprot Product Information

• NCBI GI #: 6978727
• NCBI GeneID: 25310
• NCBI Accession #: NP_036972
• NCBI GenBank Nucleotide #: NM_012840.3
• Molecular Weight: 11,743 Da
• NCBI Official Full Name: cytochrome c, testis-specific
• NCBI Official Synonym Full Names: cytochrome c, testis
• NCBI Official Symbol: Cyct
• NCBI Official Synonym Symbols: CYCTA
• NCBI Protein Information: cytochrome c, testis-specific
• UniProt Protein Name: Cytochrome c, testis-specific
• Protein Family: Cyclin
• UniProt Gene Name: Cyct
• UniProt Entry Name: CYC2_RAT

NCBI Description

testis specific cytochrome c; expressed during spermatogenesis [RGD, Feb 2006]

Uniprot Description

CYCT: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases. Belongs to the cytochrome c family

Cellular Component: mitochondrial intermembrane space

Molecular Function: electron carrier activity; metal ion binding; iron ion binding; heme binding; 3',5'-cyclic-nucleotide phosphodiesterase activity

Biological Process: apoptosis; metabolic process; signal transduction