Rabbit anti-Human HEYL Polyclonal Antibody | anti-HEYL antibody

HEYL, NT (HEYL, BHLHB33, HRT3, Hairy/enhancer-of-split related with YRPW motif-like protein, Class B basic helix-loop-helix protein 33, Hairy-related transcription factor 3) (FITC)

Cat. Num. AAA6306277

• Gene Names: HEYL; HEY3; HRT3; HESR3; bHLHb33
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HEYL; Polyclonal Antibody; NT (HEYL; BHLHB33; HRT3; Hairy/enhancer-of-split related with YRPW motif-like protein; Class B basic helix-loop-helix protein 33; Hairy-related transcription factor 3) (FITC); Hairy-related transcription factor 3; anti-HEYL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-HEYL antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: HEYL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-54 amino acids from the N-terminal region of human HEYL.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(HEYL Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HEYL antibody detected the HEYL protein (arrow).)

Immunofluorescence (IF)

(Fluorescent confocal image of Hela cell stained with HEYL Antibody (N-term).HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HEYL primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HEYL immunoreactivity is localized to Mitochondria significantly.)

Related Product Information for anti-HEYL antibody

This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The sequence of the encoded protein contains a conserved bHLH and orange domain, but its YRPW motif has diverged from other HESR family members. It is thought to be an effector of Notch signaling and a regulator of cell fate decisions. Alternatively spliced transcript variants have been found, but their biological validity has not been determined. [provided by RefSeq].

Product Categories/Family for anti-HEYL antibody

Antibodies; Transcription Factors

References

Kokubo, H., et al. Trends Cardiovasc. Med. 15(5):190-194(2005). Colland, F., et al. Genome Res. 14(7):1324-1332(2004). Iso, T., et al. J. Cell. Physiol. 194(3):237-255(2003). Nakagawa, O., et al. Proc. Natl. Acad. Sci. U.S.A. 97(25):13655-13660(2000). Firulli, B.A., et al. J. Biol. Chem. 275(43):33567-33573(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 105990530
• NCBI GeneID: 26508
• NCBI Accession #: NP_055386.1
• NCBI GenBank Nucleotide #: NM_014571
• UniProt Accession #: Q9NQ87; Q5TG99
• Molecular Weight: 35,087 Da
• NCBI Official Full Name: Homo sapiens hes-related family bHLH transcription factor with YRPW motif-like (HEYL), mRNA
• NCBI Official Synonym Full Names: hes-related family bHLH transcription factor with YRPW motif-like
• NCBI Official Symbol: HEYL
• NCBI Official Synonym Symbols: HEY3; HRT3; HESR3; bHLHb33
• NCBI Protein Information: hairy/enhancer-of-split related with YRPW motif-like protein; HEY-like protein; HRT-3; class B basic helix-loop-helix protein 33; hHRT3; hHeyL; hairy-related transcription factor 3; hairy/enhancer-of-split related with YRPW motif 3
• UniProt Protein Name: Hairy/enhancer-of-split related with YRPW motif-like protein
• Protein Family: Hairy/enhancer-of-split related with YRPW motif-like protein
• UniProt Gene Name: HEYL
• UniProt Synonym Gene Names: BHLHB33; HRT3; hHeyL; bHLHb33; HRT-3; hHRT3
• UniProt Entry Name: HEYL_HUMAN

NCBI Description

This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The sequence of the encoded protein contains a conserved bHLH and orange domain, but its YRPW motif has diverged from other HESR family members. It is thought to be an effector of Notch signaling and a regulator of cell fate decisions. Alternatively spliced transcript variants have been found, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]