Rabbit anti-Mouse Cyclin Dependent Kinase Inhibitor 2B Polyclonal Antibody | anti-CDKN2B antibody
Polyclonal Antibody to Cyclin Dependent Kinase Inhibitor 2B (CDKN2B)
Gene Names
Cdkn2b; p15; MTS2; INK4b; AV083695; p15INK4b; p15(INK4b)
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Cyclin Dependent Kinase Inhibitor 2B; Polyclonal Antibody; Polyclonal Antibody to Cyclin Dependent Kinase Inhibitor 2B (CDKN2B); p15; INK4B; MTS2; TP15; Cyclin-dependent kinase 4 inhibitor B; Multiple tumor suppressor 2; anti-CDKN2B antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CDKN2B. It has been selected for its ability to recognize CDKN2B in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence Length
138
Applicable Applications for anti-CDKN2B antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Fragment
CDKN2B (Met1~Asp130)
Conjugate
No Conjugate
Cross Reactivity
Mouse
Immunoglobulin Type
IgG
Quality Control
Content: The quality control contains recombinant CDKN2B (Met1~Asp130) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2074852
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant CDKN2B, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded intestine tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13,789 Da
NCBI Official Full Name
cyclin-dependent kinase 4 inhibitor B
NCBI Official Synonym Full Names
cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)
NCBI Official Symbol
Cdkn2b
NCBI Official Synonym Symbols
p15; MTS2; INK4b; AV083695; p15INK4b; p15(INK4b)
NCBI Protein Information
cyclin-dependent kinase 4 inhibitor B
UniProt Protein Name
Cyclin-dependent kinase 4 inhibitor B
Protein Family
UniProt Gene Name
Cdkn2b
Uniprot Description
p15-INK4B: a potent inhibitor that nteracts strongly with CDK4 and CDK6. Potential effector of TGF-beta induced cell cycle arrest.
Protein type: Cell cycle regulation; Inhibitor; Protein kinase, regulatory subunit; Tumor suppressor
Chromosomal Location of Human Ortholog: 4 C4|4 42.15 cM
Cellular Component: cytoplasm; nucleus
Molecular Function: cyclin-dependent protein serine/threonine kinase inhibitor activity; protein kinase binding
Biological Process: cell cycle; cell cycle arrest; cellular response to extracellular stimulus; cellular response to nutrient; G2/M transition of mitotic cell cycle; mitotic cell cycle checkpoint; negative regulation of cell cycle G1/S phase transition; negative regulation of cell proliferation; negative regulation of epithelial cell proliferation; negative regulation of G1/S transition of mitotic cell cycle; negative regulation of phosphorylation; positive regulation of transforming growth factor beta receptor signaling pathway; regulation of cyclin-dependent protein kinase activity; spleen development
