Typical Testing Data/Standard Curve (for reference only)
Mouse Aldehyde dehydrogenase family 1 member A3 ELISA Kit | ALDH1A3 elisa kit
Mouse Aldehyde dehydrogenase family 1 member A3 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ADP enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for ADP. Standards or samples are then added to the microtiter plate wells and ADP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ADP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ADP are added to each well to "sandwich" the ADP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain ADP and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an aldehyde dehydrogenase enzyme that uses retinal as a substrate. Mutations in this gene have been associated with microphthalmia, isolated 8, and expression changes have also been detected in tumor cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
Uniprot Description
ALDH1A3: Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Seems to be the key enzyme in the formation of an RA gradient along the dorso-ventral axis during the early eye development and also in the development of the olfactory system. Belongs to the aldehyde dehydrogenase family.
Protein type: Xenobiotic Metabolism - metabolism by cytochrome P450; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Oxidoreductase; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - histidine; Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 1.2.1.5; Amino Acid Metabolism - tyrosine
Chromosomal Location of Human Ortholog: 15q26.3
Cellular Component: cytoplasm; cytosol
Molecular Function: aldehyde dehydrogenase (NAD) activity; protein homodimerization activity; aldehyde dehydrogenase [NAD(P)+] activity; retinal dehydrogenase activity
Biological Process: inner ear morphogenesis; retinal metabolic process; retinol metabolic process; embryonic eye morphogenesis; positive regulation of apoptosis; righting reflex; olfactory pit development; optic cup morphogenesis involved in camera-type eye development; retinoic acid metabolic process; locomotory behavior; neuromuscular process controlling balance; nucleus accumbens development
Disease: Microphthalmia, Isolated 8
Research Articles on ALDH1A3
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Product Notes
The Mouse ALDH1A3 aldh1a3 (Catalog #AAA732873) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA732873 ELISA Kit recognizes Mouse ALDH1A3. It is sometimes possible for the material contained within the vial of "Aldehyde dehydrogenase family 1 member A3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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