Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Cyclin Dependent Kinase Inhibitor 1B Polyclonal Antibody | anti-CDKN1B antibody
Polyclonal Antibody to Cyclin Dependent Kinase Inhibitor 1B (CDKN1B)
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
NCBI and Uniprot Product Information
Uniprot Description
p27Kip1: a cell-cycle regulatory protein that Interacts with cyclin-CDK2 and -CDK4, inhibiting cell cycle progression at G1. May mediate TGF beta-induced g1 arrest. Its degradation is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
Protein type: Cell cycle regulation; Inhibitor; Oncoprotein; Protein kinase, regulatory subunit
Chromosomal Location of Human Ortholog: 6 G1|6 65.77 cM
Cellular Component: cytoplasm; cytosol; endosome; intracellular membrane-bound organelle; nucleus; protein complex
Molecular Function: chaperone binding; cyclin binding; cyclin-dependent protein kinase activity; cyclin-dependent protein serine/threonine kinase inhibitor activity; Hsp70 protein binding; protein binding; protein complex binding; protein kinase binding; protein kinase inhibitor activity; protein phosphatase binding
Biological Process: autophagic cell death; cell cycle; cell cycle arrest; cell death; cellular response to lithium ion; cellular response to organic cyclic compound; G1/S transition of mitotic cell cycle; inner ear development; mitotic cell cycle arrest; negative regulation of apoptosis; negative regulation of cell cycle; negative regulation of cell growth; negative regulation of cell motility; negative regulation of cell proliferation; negative regulation of cyclin-dependent protein kinase activity; negative regulation of cyclin-dependent protein serine/threonine kinase activity; negative regulation of epithelial cell proliferation; negative regulation of kinase activity; negative regulation of mitotic cell cycle; negative regulation of phosphorylation; negative regulation of transcription, DNA-dependent; negative regulation of vascular smooth muscle cell proliferation; Notch signaling pathway; placenta development; positive regulation of cell death; positive regulation of cell proliferation; positive regulation of cyclin-dependent protein serine/threonine kinase activity; positive regulation of microtubule polymerization; potassium ion transport; regulation of cell proliferation; regulation of exit from mitosis; regulation of lens fiber cell differentiation; sensory perception of sound
Research Articles on CDKN1B
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Product Notes
The CDKN1B cdkn1b (Catalog #AAA2033881) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Cyclin Dependent Kinase Inhibitor 1B (CDKN1B) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclin Dependent Kinase Inhibitor 1B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-1:5000. Researchers should empirically determine the suitability of the CDKN1B cdkn1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclin Dependent Kinase Inhibitor 1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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