Rabbit Cullin 5 Polyclonal Antibody | anti-CUL5 antibody

Anti-Cullin 5 Picoband antibody

Cat. Num. AAA1750730

• Gene Names: CUL5; CUL-5; VACM1; VACM-1
• Reactivity: Human, Mouse, Rat; No cross reactivity with other proteins.
• Applications: ELISA, Western Blot
• Synonyms: Cullin 5; Polyclonal Antibody; Anti-Cullin 5 Picoband antibody; Cullin-5; CUL-5; Vasopressin-activated calcium-mobilizing receptor 1; VACM-1; CUL5; VACM1; anti-CUL5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; No cross reactivity with other proteins.
• Clonality: Polyclonal
• Form/Format: Lyophilized
• Sequence Length: 780

• Applicable Applications for anti-CUL5 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 0.1-0.5mug/ml; Direct ELISA: 0.1-0.5mug/ml
• Immunogen: E Coli-derived human Cullin 5 recombinant protein (Position: A2-D105).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Figure 1. Western blot analysis of Cullin 5 using anti-Cullin 5 antibody (MBS1750730).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: rat spleen tissue lysates,Lane 3: rat heart tissue lysates,Lane 4: rat kidney tissue lysates,Lane 5: mouse brain tissue lysates,Lane 6: mouse spleen tissue lysates,Lane 7: mouse heart tissue lysates,Lane 8: mouse kidney tissue lysates,Lane 9: mouse testis tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cullin 5 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cullin 5 at approximately 91KD. The expected band size for Cullin 5 is at 91KD. )

Related Product Information for anti-CUL5 antibody

Description: Cullin-5 is a protein that in humans is encoded by the CUL5 gene. Cul5 inhibits cellular proliferation, potentially through its involvement in the SOCS/ BC-box/ eloBC/ cul5/ RING E3 ligase complex, which functions as part of the ubiquitin system for protein degradation. One study have shown that Cul5 plays a role in Reelin signaling cascade, participating in the DAB1 degradation and thus ensuring the negative feedback mechanism of Reelin signaling during corticogenesis.
Protein Function: Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin- conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor.

NCBI and Uniprot Product Information

• NCBI GI #: 40254446
• NCBI GeneID: 8065
• NCBI Accession #: NP_003469.2
• NCBI GenBank Nucleotide #: NM_003478.4
• UniProt Accession #: Q93034; O14766; Q9BZC6; A8K960
• Molecular Weight: 90,955 Da
• NCBI Official Full Name: cullin-5
• NCBI Official Synonym Full Names: cullin 5
• NCBI Official Symbol: CUL5
• NCBI Official Synonym Symbols: CUL-5; VACM1; VACM-1
• NCBI Protein Information: cullin-5
• UniProt Protein Name: Cullin-5
• Protein Family: Cullin
• UniProt Gene Name: CUL5
• UniProt Synonym Gene Names: VACM1; CUL-5; VACM-1

Uniprot Description

Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor.