Western Blot (WB)
(SMG5 Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the SMG5 antibody detected the SMG5 protein (arrow).)
Rabbit SMG5 Polyclonal Antibody | anti-SMG5 antibody
SMG5 Antibody (C-term)
Gene Names
SMG5; EST1B; SMG-5; LPTSRP1; LPTS-RP1
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SMG5; Polyclonal Antibody; SMG5 Antibody (C-term); Protein SMG5; EST1-like protein B; LPTS-RP1; LPTS-interacting protein; SMG-5 homolog; hSMG-5; EST1B; KIAA1089; anti-SMG5 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SMG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 912-941 amino acids from the C-terminal region of human SMG5.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.34 (varies by lot)
Sequence Positions
912-941
Sequence Length
1016
Applicable Applications for anti-SMG5 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(SMG5 Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the SMG5 antibody detected the SMG5 protein (arrow).)
Western Blot (WB)
(SMG5 Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the SMG5 antibody detected the SMG5 protein (arrow).)
Related Product Information for anti-SMG5 antibody
SMG5 is involved in nonsense-mediated mRNA decay (Ohnishi
et al., 2003 [PubMed 14636577]).
et al., 2003 [PubMed 14636577]).
References
Kamatani, Y., et al. Nat. Genet. 42(3):210-215(2010)
Glavan, F., et al. EMBO J. 25(21):5117-5125(2006)
Lee, H., et al. Mol. Cell. Biol. 26(14):5259-5269(2006)
Azzalin, C.M., et al. Curr. Biol. 16(4):433-439(2006)
Unterholzner, L., et al. Mol. Cell 16(4):587-596(2004)
Glavan, F., et al. EMBO J. 25(21):5117-5125(2006)
Lee, H., et al. Mol. Cell. Biol. 26(14):5259-5269(2006)
Azzalin, C.M., et al. Curr. Biol. 16(4):433-439(2006)
Unterholzner, L., et al. Mol. Cell 16(4):587-596(2004)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
113928
NCBI Official Full Name
protein SMG5
NCBI Official Synonym Full Names
SMG5 nonsense mediated mRNA decay factor
NCBI Official Symbol
SMG5
NCBI Official Synonym Symbols
EST1B; SMG-5; LPTSRP1; LPTS-RP1
NCBI Protein Information
protein SMG5
UniProt Protein Name
Protein SMG5
Protein Family
UniProt Gene Name
SMG5
UniProt Synonym Gene Names
EST1B; KIAA1089; hSMG-5
UniProt Entry Name
SMG5_HUMAN
NCBI Description
SMG5 is involved in nonsense-mediated mRNA decay (Ohnishi et al., 2003 [PubMed 14636577]).[supplied by OMIM, Mar 2008]
Uniprot Description
SMG5: Plays a role in nonsense-mediated mRNA decay. Does not have RNase activity by itself. Promotes dephosphorylation of UPF1. Together with SMG7 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. Necessary for TERT activity.
Chromosomal Location of Human Ortholog: 1q21.2
Cellular Component: cytoplasm; cytosol; nucleus
Molecular Function: protein binding; protein phosphatase 2A binding
Biological Process: mRNA export from nucleus; mRNA catabolic process, nonsense-mediated decay; regulation of dephosphorylation; gene expression
Research Articles on SMG5
Similar Products
Product Notes
The SMG5 smg5 (Catalog #AAA9210133) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 912-941. The SMG5 Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's SMG5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the SMG5 smg5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMG5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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