Western Blot (WB)
(CPSF3L Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CPSF3L antibody detected the CPSF3L protein (arrow).)
Rabbit anti-Human CPSF3L Polyclonal Antibody | anti-CPSF3L antibody
CPSF3L, NT (CPSF3L, INTS11, RC68, Integrator complex subunit 11, Cleavage and polyadenylation-specific factor 3-like protein, Protein related to CPSF subunits of 68kD)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Immunofluorescence: 1:10-50
Western Blot (WB)
(CPSF3L Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CPSF3L antibody detected the CPSF3L protein (arrow).)
Western Blot (WB)
(CPSF3L Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CPSF3L antibody detected the CPSF3L protein (arrow).)
Immunohistochemistry (IHC)
(CPSF3L Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CPSF3L Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(CPSF3L Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CPSF3L Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of CPSF3L Antibody (N-term) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of CPSF3L Antibody (N-term) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
NCBI and Uniprot Product Information
NCBI Description
The Integrator complex contains at least 12 subunits and associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates the 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690). INTS11, or CPSF3L, is the catalytic subunit of the Integrator complex (Baillat et al., 2005 [PubMed 16239144]).[supplied by OMIM, Mar 2008]
Uniprot Description
Function: Catalytic component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates the snRNAs 3' cleavage. Ref.7
Subunit structure: Belongs to the multiprotein complex Integrator, at least composed of INTS1, INTS2, INTS3, INTS4, INTS5, INTS6, INTS7, INTS8, INTS9/RC74, INTS10, CPSF3L/INTS11 and INTS12. Ref.7
Subcellular location: Nucleus. Cytoplasm Ref.8.
Domain: The HXHXDH motif is essential for the endoribonuclease activity of the CPSF complex
By similarity.
Sequence similarities: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. INTS11 subfamily.
Sequence caution: The sequence BAA91246.1 differs from that shown. Reason: Frameshift at position 587. The sequence BAB14541.1 differs from that shown. Reason: Frameshift at position 248. The sequence CAI23174.1 differs from that shown. Reason: Erroneous initiation. The sequence CAI23176.1 differs from that shown. Reason: Erroneous initiation. The sequence CAI23177.1 differs from that shown. Reason: Erroneous initiation. The sequence CAI23179.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI23180.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI23181.1 differs from that shown. Reason: Erroneous gene model prediction.
Research Articles on CPSF3L
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Product Notes
The CPSF3L cpsf3l (Catalog #AAA649795) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CPSF3L, NT (CPSF3L, INTS11, RC68, Integrator complex subunit 11, Cleavage and polyadenylation-specific factor 3-like protein, Protein related to CPSF subunits of 68kD) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CPSF3L can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50 Immunofluorescence: 1:10-50. Researchers should empirically determine the suitability of the CPSF3L cpsf3l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CPSF3L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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