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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Human Testis Specific Kinase 1 Polyclonal Antibody | anti-TESK1 antibody

Testis Specific Kinase 1 (TESK1) Polyclonal Antibody

Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Testis Specific Kinase 1; Polyclonal Antibody; Testis Specific Kinase 1 (TESK1) Polyclonal Antibody; Dual Specificity Testis-Specific Protein Kinase 1; anti-TESK1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against TESK1. It has been selected for its ability to recognize TESK1 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
0.57mg/ml (varies by lot)
Applicable Applications for anti-TESK1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Human
Organism Species
Human
Fragment
TESK1 (Phe57~Ile314)
Quality Control
Content: The quality control contains recombinant TESK1 (Phe57~Ile314) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2068219
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded liver tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded liver tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
67,684 Da
NCBI Official Full Name
dual specificity testis-specific protein kinase 1 isoform 2
NCBI Official Synonym Full Names
testis-specific kinase 1
NCBI Official Symbol
TESK1
NCBI Protein Information
dual specificity testis-specific protein kinase 1
UniProt Protein Name
Dual specificity testis-specific protein kinase 1
UniProt Gene Name
TESK1

NCBI Description

This gene product is a serine/threonine protein kinase that contains an N-terminal protein kinase domain and a C-terminal proline-rich domain. Its protein kinase domain is most closely related to those of the LIM motif-containing protein kinases (LIMKs). The encoded protein can phosphorylate myelin basic protein and histone in vitro. The testicular germ cell-specific expression and developmental pattern of expression of the mouse gene suggests that this gene plays an important role at and after the meiotic phase of spermatogenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]

Uniprot Description

TESK1: a TKL kinase of the LISK fanily. Its testicular germ cell-specific expression and developmental pattern of expression in the mouse suggests that this kinase plays an important role at and after the meiotic phase of spermatogenesis.

Protein type: EC 2.7.12.1; Kinase, protein; LISK family; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TESK subfamily; TKL group

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: cytoplasm; cytoplasmic vesicle; cytosol

Molecular Function: ATP binding; metal ion binding; protein binding; protein C-terminus binding; protein kinase binding; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity; protein tyrosine kinase activity; signal transducer activity

Biological Process: negative regulation of protein autophosphorylation; negative regulation of protein serine/threonine kinase activity; peptidyl-tyrosine phosphorylation; positive regulation of stress fiber formation; regulation of protein localization; spermatogenesis

Research Articles on TESK1

Similar Products

Product Notes

The TESK1 tesk1 (Catalog #AAA2032734) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Testis Specific Kinase 1 (TESK1) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Testis Specific Kinase 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the TESK1 tesk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Testis Specific Kinase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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