Western Blot (WB)
(Figure 1 Western Blot Validation with SARS-CoV-2 (COVID-19) Spike S2 ECD Recombinant ProteinLoading: 30 ng per lane of SARS-CoV-2 (COVID-19) Spike S2 ECD recombinant protein, 10-115. Antibodies: SARS-CoV-2 (COVID-19) Spike S2, 9119, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 μg/mL and Lane 2: 2 μg/mL.)
Rabbit COVID 19 Spike S2 Coronavirus Polyclonal Antibody | anti-COVID-19 antibody
SARS-CoV-2 (COVID-19) Spike S2 Antibody
Reactivity
Virus
Applications
ELISA, Western Blot, Immunofluorescence
Purity
SARS-CoV-2 (COVID-19) Spike Antibody is affinity chromatography purified via peptide column
Synonyms
COVID 19 Spike S2 Coronavirus; Polyclonal Antibody; SARS-CoV-2 (COVID-19) Spike S2 Antibody; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike S2; anti-COVID-19 antibody
Host
Rabbit
Reactivity
Virus
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
SARS-CoV-2 (COVID-19) Spike Antibody is affinity chromatography purified via peptide column
Form/Format
Liquid
SARS-CoV-2 (COVID-19) Spike Antibody is supplied in PBS containing 0.02% sodium azide.
SARS-CoV-2 (COVID-19) Spike Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-COVID-19 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1ug/mL
IF: 10ug/mL.
Antibody validated: Immunofluorescence in human samples. SARS-CoV-2 (COVID-19) Spike antibody can be used for the detection of SARS-CoV-2 (COVID-19) Spike protein in ELISA. It will detect 4 ng of free peptide at 1ug/mL. All other applications and species not yet tested.
IF: 10ug/mL.
Antibody validated: Immunofluorescence in human samples. SARS-CoV-2 (COVID-19) Spike antibody can be used for the detection of SARS-CoV-2 (COVID-19) Spike protein in ELISA. It will detect 4 ng of free peptide at 1ug/mL. All other applications and species not yet tested.
Source
SARS-CoV-2
Immunogen
Anti-SARS-CoV-2 (COVID-19) Spike antibody (9119) was raised against a peptide corresponding to 16 amino acids near the center terminus of SARS-CoV-2 (COVID-19) Spike glycoprotein.
The immunogen is located within 900-950 amino acids of SARS-CoV-2 (COVID-19) Spike protein.
The immunogen is located within 900-950 amino acids of SARS-CoV-2 (COVID-19) Spike protein.
Homology
Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (75%)
Isoforms
SARS-CoV-2 (COVID-19) Spike has one isoform (1273aa)
Conjugation
Unconjugated
Preparation and Storage
SARS-CoV-2 (COVID-19) Spike antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Figure 1 Western Blot Validation with SARS-CoV-2 (COVID-19) Spike S2 ECD Recombinant ProteinLoading: 30 ng per lane of SARS-CoV-2 (COVID-19) Spike S2 ECD recombinant protein, 10-115. Antibodies: SARS-CoV-2 (COVID-19) Spike S2, 9119, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 μg/mL and Lane 2: 2 μg/mL.)
Western Blot (WB)
(Figure 1 Western Blot Validation with SARS-CoV-2 (COVID-19) Spike S2 ECD Recombinant ProteinLoading: 30 ng per lane of SARS-CoV-2 (COVID-19) Spike S2 ECD recombinant protein, 10-115. Antibodies: SARS-CoV-2 (COVID-19) Spike S2, 9119, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 μg/mL and Lane 2: 2 μg/mL.)
Related Product Information for anti-COVID-19 antibody
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019-20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a Spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. Coronavirus invades cells through Spike (S) glycoproteins, a class I fusion protein. It is the major viral surface protein that coronavirus uses to bind to the human cell surface receptor. It also mediates the fusion of host and viral cell membrane, allowing the virus to enter human cells and begin infection (3). The spike protein is the major target for neutralizing antibodies and vaccine development (4). The protein modeling suggests that there is strong interaction between Spike protein receptor-binding domain and its host receptor angiotensin-converting enzyme 2 (ACE2), which regulate both the cross-species and human-to-human transmissions of COVID-19 (5). The recent study has shown that the SARS-CoV-2 spike protein binds ACE2 with higher affinity than SARS-CoV spike protein (6).
Product Categories/Family for anti-COVID-19 antibody
References
Gorbalenya. bioRxiv: 2020.
Hui et al. Int J Infect Dis. 2020;91:264-266.
Belouzard et al. Viruses. 2012;4(6):1011-33.
Lee et al. J Virol. 2006;80(8):4079-87.
Wan et al. J Virol. 2020.
Hui et al. Int J Infect Dis. 2020;91:264-266.
Belouzard et al. Viruses. 2012;4(6):1011-33.
Lee et al. J Virol. 2006;80(8):4079-87.
Wan et al. J Virol. 2020.
NCBI and Uniprot Product Information
Research Articles on COVID-19
Similar Products
Product Notes
The COVID-19 (Catalog #AAA154656) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SARS-CoV-2 (COVID-19) Spike S2 Antibody reacts with Virus and may cross-react with other species as described in the data sheet. AAA Biotech's COVID 19 Spike S2 Coronavirus can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). WB: 1ug/mL IF: 10ug/mL. Antibody validated: Immunofluorescence in human samples. SARS-CoV-2 (COVID-19) Spike antibody can be used for the detection of SARS-CoV-2 (COVID-19) Spike protein in ELISA. It will detect 4 ng of free peptide at 1ug/mL. All other applications and species not yet tested. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 Spike S2 Coronavirus, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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